Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (29): 5412-5416.doi: 10.3969/j.issn.2095-4344.2012.29.019

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Vascular endothelial regeneration after small-diameter bioartificial vascular graft

Lin Lü-biao1, Xu Yi-min1, Qi Song-tao2, Zeng Shao-wen3, Li Wei-qiu4, Zhuang Bing-rong4   

  1. 1Department of Neurosurgery, First Affiliated Hospital of Shantou University Medical College, Shantou 515041, Guangdong Province, China;
    2Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province China;
    3Department of Pathology, Central Hospital of Shantou City, Shantou 515041, Guangdong Province China;
    4Center Laboratory, Shantou University Medical College, Shantou 515041, Guangdong Province , China
  • Received:2012-01-17 Revised:2012-04-13 Online:2012-07-15 Published:2012-07-15
  • Contact: Xu Yi-min, Doctor, Associate chief physician, Department of Neurosurgery, First Affiliated Hospital of Shantou University Medical College, Shantou 515041, Guangdong Province, China xuym@163.com
  • About author:Lin Lü-biao☆, Doctor, Associate chief physician, Department of Neurosurgery, First Affiliated Hospital of Shantou University Medical College, Shantou 515041, Guangdong Province, China linlvbiao@sina.com

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Abstract:

BACKGROUND: Characteristics of vascular prostheses are similar to the natural vessels and it has high histocompatibility after transplantation. But endothelial regeneration is the key for vascular prosthesis unblocked eternally.
OBJECTIVE: To investigate the histocompatibility of a novel small-diameter bioartificial vascular graft at different periods postoperatively and the histopathologic changes following intimal regeneration of the vessel wall.
METHODS: A dog model of artificial vascular grafts implanted end-to-end by a continuous suture in the common carotid artery was established.
RESULTS AND CONCLUSION: ①Light microscope results showed that at week 12 after graft, endothelial cells were sparsely distributed throughout the neointima surface near the stoma; at month 6 after graft, endothelial cells were presented on the whole surface of the patent graft lumen; 1.5 years after graft, lumens of graft vessels were unobstructed, and parts of endomembrane tissues were found chronic inflammation. ②Electron microscope results showed that at week 12 after graft, new vascular endothelial cells were in fusiform shape and arranged regularly; besides, the endothelial cells spread from the stoma to the middle of the graft vessel. After graft for 6 months, the endothelial cells spread from the stoma to the middle of the graft vessel. Endothelial cell colony in the middle of the graft vessel showed leaping and flake-like growth, and the cells were arranged more compactly; moreover, their shapes were more like the endothelial cells of mature vessels. After graft for 1.5 years, the whole vessel wall was covered by tightly arranged endothelial cells, and parts of endomembrane tissues revealed chronic inflammation. These findings suggest that the novel small-diameter bioartificial vascular graft has many advantages, for example, the newborn intima forms early and completely, and endangium can be well reconstructed; in addition, it has good biocompatibility and stability.

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