Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (27): 5076-5079.doi: 10.3969/j.issn.1673-8225.2011.27.030

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Effect of GinsenosideRb1 and beta-amyloid 25-35 on tau phosphorylation in differentiated neural stem cells

Zhao Qing-xia1, Liu Xiao-zhuan2, Li Bo3, Yan Wen-hai3, Han Xue-fei3, Xing Ying3   

  1. 1Nursing College of Zhengzhou University, Zhengzhou  450053, Henan Province, China; 3Stem Cells Research Center of Zhengzhou University, Zhengzhou  450053, Henan Province, China; 2Medical College, Henan University of Science and Technology, Zhengzhou  450053, Henan Province, China
  • Received:2011-01-26 Revised:2011-04-25 Online:2011-07-02 Published:2011-07-02
  • Contact: Xing Ying, Doctor, Professor, Stem Cells Research Center of Zhengzhou University, Zhengzhou 450053, Henan Province, China xingy@zzu.edu.cn
  • About author:Zhao Qing-xia★, Master, Nursing College of Zhengzhou University, Zhengzhou 450053, Henan Province, China zzuzhaoqingxia@126.com Liu Xiao-zhuan, Master, Medical College, Henan University of Science and Technology, Zhengzhou 450053, Henan Province, China anya0609@126.com Zhao Qing-xia and Liu Xiao-zhuan contributed equally to this paper.
  • Supported by:

    the Science and Technology Tackle Key Foundation of Henan Province, No. 0424410085*; the Natural Science Foundation of Henan Province, No. 0611043000*

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Abstract:

BACKGROUND: Currently, neural stem cells (NSCs) transplantation effect on the treatment of Alzheimer’s disease is improved by adjusting tau protein phosphorylation level during the differentiation of NSCs.
OBJECTIVE: To explore the effect of GinsenosideRb1 and beta-amyloid 25-35 (Aβ25-35) on tau phosphorylation after NSCs are transformed into neurons.
METHODS: NSCs were isolated and cultured from rat hippocampus. NSCs of the third passage were induced towards neurons after one week; the expression of Tau[pS396], Tau[pS262] and GSK-3β[pTyr279, 216] were tested by the immunofluorescent cytochemical staining and western-blot.
RESULTS AND CONCLUSION: The expression of Tau[pS396], Tau[pS262] and GSK-3β[pTyr279, 216] was found in the process of NSCs differentiation. Aβ25-35 could enhance their expression; moreover Ginsenoside Rb1 could reverse the effect of Aβ25-35. The results indicated that there was a certain level of phosphorylation in tau protein during NSCs differentiation. Aβ25-35 and GinsenosideRb1 could regulate the level of tau phosphorylation. The mechanism of its action is by adjusting GSK-3β activity.

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