Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (27): 5068-5071.doi: 10.3969/j.issn.1673-8225.2011.27.028

Previous Articles     Next Articles

Mannitol improves the efficacy of intravenous transplantation of human umbilical cord blood CD34+ cells for cerebral infarction in hypertensive rats

Yu Guo-long, Ou Ya-li, Yang Tian-lun, Fang Li, Hu Ke   

  1. Department of Cardiology, Xiangya Hospital of Central South University, Changsha  410008, Hunan Province, China
  • Received:2011-01-11 Revised:2011-04-29 Online:2011-07-02 Published:2011-07-02
  • About author:Yu Guo-long☆, Doctor, Professor, Department of Cardiology, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China yuguolong123@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30572079*

PDF

245

Abstract:

BACKGROUND: Intravenous transplantation of human umbilical cord blood cells (HUCBCs) in treatment of cerebral infarction is limited. Only a small fraction of the grafted cells appears to survive at the damaged brain and the behavioral recovery is only partial.
OBJECTIVE: To evaluate the ability of Mannitol, a blood brain barrier (BBB) permeabilizer, to enhance neuroprotective effects of intravenously administered HUCBCs for cerebral infarction in spontaneous hypertensive rats.
METHODS: CD34+ cells isolated from HUCB were transfected by GFP plasmid with liposome method. Forty-five adult male spontaneous hypertensive rats with middle cerebral artery occlusion were randomly divided into three groups at 24 hours after modeling, each of 15: Experimental group, GFP-CD34+ cells (1×106) and 20% Mannitol (2 g/kg) were administrated intravenously at 24 hours after modeling; Positive control group, GFP-CD34+ cells (1×106) were only injected intravenously at same time; Blank control group, normal saline was injected at same time.
RESULTS AND CONCLUSION: ①The number of GFP positive cells in cerebral tissues in the experimental group was increased significantly compared to the positive control group. ②No significant difference was found in the neurologic function scores at day 7 after transplantation among the three groups; but at day 28 after transplantation, the neurologic function in the experimental group was improved significantly compared with the positive or blank control group (P < 0.05). ③The cerebral infarct volume in the experimental group was significantly decreased compared with the positive or blank control group. ④The glial cell line-derived neurotrophic factor level in cerebral tissue in the experimental group was significantly increased compared with the positive and blank control groups. The results suggested that Mannitol enhanced CD34+ cells to migrate into ischemic-injured cerebral tissues, and to improve the efficacy of CD34+ cells delivered intravenously for cerebral infarction in spontaneous hypertensive rats.

CLC Number: