Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (27): 5058-5062.doi: 10.3969/j.issn.1673-8225.2011.27.026

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In vitro effect of stromal cell derived factor -1 on the migration of neural stem cells

Mu Lin-jie, Ding Peng, Wang Chong-qian, Wang Wei-min, Li Xuan-peng, Wang Jin-kun   

  1. Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China
  • Received:2011-01-16 Revised:2011-03-24 Online:2011-07-02 Published:2011-07-02
  • Contact: Wang Jin-kun, Chief physician, Master’s supervisor, Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China
  • About author:Mu Lin-jie★, Studying for master’s degree, Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China mulinjie84@gmail.com Ding Peng☆, Doctor, Associate professor, Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China Mu Lin-jie and Ding Peng contributed equally to this paper.
  • Supported by:

    Applied Basic Research of Yunnan Province in 2008, No. 2008ZC132M*

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Abstract:

BACKGROUND: Neural stem cell migration plays important roles in the development and repair of the central nervous system. Although recent studies have shown that the chemokines mediate neural stem cell migration, but the mechanism is unclear.
OBJECTIVE: To explore the effects of stromal cell-derived factor-1 (SDF-1) on migration of fetal rat hippocampus neural stem cells in vitro.
METHODS: The fetal rat hippocampal neural stem cells were isolated, cultured and identified in serum-free medium. The expression of CXCR4 in neural stem cells was detected using immunofluorescence and RT-PCR. The under-agarose cell migration assay was used to observe the effects of SDF-1(50-500 ng/ml) on neural stem cell migration, and blocking CXCR4, the receptor of SDF-1, to identify the specificity of migration.
RESULTS AND CONCLUSION: Immunofluorescence results showed that neural stem cells were CXCR4 positive, and RT-PCR findings showed 643 bp specific band in agarose gel electrophoresis. The Under-Agarose cell migration assay showed that: SDF-1 (50-500 ng/ml) could accelerate the migration of neural stem cells, the migration increased with the concentration, and 500 μg/L was the best. By adding anti-CXCR4 polyclonal antibodies, the migration of neural stem cells compared with SDF-1 was significantly reduced, no significant difference with the control group (P > 0.05), pointing out anti-CXCR4 polyclonal antibodies can block the effect of the migration.

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