Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (27): 5053-5057.doi: 10.3969/j.issn.1673-8225.2011.27.025

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Hemeoxygenase-1 expression effect on the up-regulation of peripheral blood CD4+CD25+CD127low/- regulatory T cells mediated by mesenchymal stem cells in asthma patients 

Wen Bing1, Zhuansun Yong-xun1, Yan Fu-de2, Chen Rui1, Zhang Wei1, Feng Su-ling1, Li Jian-guo1   

  1. 1Department of Respiratory Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou  510120, Guangdong Province, China
    2Department of Internal Medicine, Dashi People’s Hospital, Guangzhou  511430, Guangdong Province, China
  • Received:2011-01-27 Revised:2011-05-21 Online:2011-07-02 Published:2011-07-02
  • Contact: Li Jian-guo, Doctor, Professor, Master’s supervisor, Department of Respiratory Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China jgli88@126.com
  • About author:Wen Bing★, studying for master’s degree, Department of Respiratory Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China wenljh@163.com
  • Supported by:

    the Science and Technology Program of Guangdong Province, No. 2010B031600082*

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Abstract:

BACKGROUND: Up-regulating CD4+CD25+CD127low/- regulatory T cells is a new target in the treatment of asthma. Human bone marrow mesenchymal stem cells (MSCs) can up-regulate CD4+CD25+CD127low/- regulatory T cells in vitro, while the mechanism is not clear.
OBJECTIVE: To study the effect of hemeoxygenase-1 (HO-1) expression in the up-regulation of peripheral blood CD4+CD25+CD127low/- regulatory T cells mediated by MSCs in asthma patients.
METHODS: Real-time PCR was used to examine the expression of HO-1 mRNA in MSCs pretreated with 0, 15, 30, 45, 60 μmol/L Hemin (the revulsive of HO-1) and 0, 5, 10, 15, 20 μmol/L ZnPP (the inhibitor of HO-1) respectively. Peripheral blood mononuclear cells (PBMCs), which were isolated from 10 cases of asthma patients with acute episode and 10 cases of healthy controls using Ficoll density gradient centrifugation, were incubated with MSCs pretreated with Hemin, ZnPP and mock respectively.
RESULTS AND CONCLUSION: The expression of HO-1 in MSCs can be induced and inhibited in vitro. The higher concentration of Hemin added to MSCs, the higher expression of HO-1 mRNA was tested (P < 0.05). With the increasing concentration of ZnPP added to MSCs, the expression of HO-1 mRNA was becoming lower (P < 0.05). The proportion of CD4+CD25+CD127low/- regulatory T cells in CD4+ T cells could be up-regulated by MSCs (P < 0.01) and also by MSCs with induction of HO-1 expression (P < 0.01). While MSCs with inhibition of HO-1 expression could down-regulate the proportion of CD4+CD25+CD127low/-regulatory T cells in CD4+ T cells (P < 0.01). The expression of HO-1 partially contributed to the up-regulation of CD4+CD25+CD127low/- regulatory T cells mediated by MSCs in asthma patients.

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