Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (27): 5048-5052.doi: 10.3969/j.issn.1673-8225.2011.27.024

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Differentiation of rabbit mesenchymal stem cells into cartilage phenotype induced by transforming growth factor beta 3  

Sui Lai-jian1, Lü Cheng-yu1, Zhang Hai-ning1, Xu Hao1, Gao Zheng-yu2, Sui Ai-hua3, Wang Ying-zhen1   

  1. 1Department of Joint Surgery, 2Department of Rehabilitation, 3Central Laboratory, Affiliated Hospital, Qingdao University Medical College, Qingdao  266000, Shandong Province, China
  • Received:2011-01-03 Revised:2011-02-24 Online:2011-07-02 Published:2011-07-02
  • Contact: Wang Ying-zhen, Doctoral supervisor, Department of Joint Surgery, Affiliated Hospital, Qingdao University Medical College, Qingdao 266000, Shandong Province, China wangyingzhenqd@163.com
  • About author:Sui Lai-jian★, Master, Department of Joint Surgery, Affiliated Hospital, Qingdao University Medical College, Qingdao 266000, Shandong Province, China
  • Supported by:

    the Science and Technology Tackle Key Plan of Qingdao City, No.06-2-2-6- nsh-2*

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Abstract:

BACKGROUND: Endogenous induction of cartilage differentiation is to use a certain carrier to integrate the target genes into stem cells to induce self-differentiation by self-induced secretion of cytokines.
OBJECTIVE: To observe the ability of rabbit bone marrow mesenchymal stem cells (BMSCs) transfected with transforming growth factor beta 3 (TGF-β3) to differentiate into cartilage phenotype through adeno-associated virus vector.
METHODS: The third passage BMSCs in logarithmic growth phase was transfected by rAAV2-TGF-β3. Total protein was extracted from cells at 3, 6, 9, 12 days after transfection, and TGF-β3 was detected through ELISA. After 2 weeks of culturing, mRNA expressions of type Ⅱ collagen were determined by RT-PCR and the collagen Ⅱ protein was detected by western blot. The expression of proteoglycan was shown by toluidine blue staining.
RESULTS AND CONCLUSION: After transfection, BMSCs can express TGF-β3 stably, and make a better cartilage transformation compared to negative control group. TGF-β3 can be transfected into BMSCs through adeno-associated virus vectors and induce cell differentiation into cartilage phenotype.

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