Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (27): 5039-5043.doi: 10.3969/j.issn.1673-8225.2011.27.022

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Effect of peripheral blood mesenchymal stem cells transplantation on neovascularization and cardiac function of myocardial infarction rabbits

Liu Zhi-jiang, Shi Bei, Zhao Ran-zun, Shen Chang-ying, Wang Dong-mei, Wang Zheng-long   

  1. Department of Cardiology, the First Affiliated Hospital of Zunyi Medical College, Zunyi  563003, Guizhou Province, China
  • Received:2010-12-20 Revised:2011-03-04 Online:2011-07-02 Published:2011-07-02
  • Contact: Shi Bei, Master, Professor, Master’s supervisor, Department of Cardiology, the First Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China shibei2147@yahoo.com.cn
  • About author:Liu Zhi-jiang★, Master, Attending physician, Department of Cardiology, the First Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China liuzhijiang348@126.com

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Abstract:

BACKGROUND: Both Drug treatment and percutaneous coronary intervention cannot repair the necrotic myocardium following myocardial infarction.
OBJECTIVE: To investigate the effect of peripheral blood mesenchymal stem cells (MSCs) transplantation on the neovascularization and cardiac function following myocardial infarction in rabbits.
METHODS: Thirty-six New Zealand rabbits were divided into MSCs transplantation group (n=12), control group (n=12) and sham operation group (n=12). Rabbits models of acute myocardial infarction were established in the former two groups.
RESULTS AND CONCLUSION: Flow cytometry demonstrated that most MSCs expressed CD44, very few cells expressed CD34 and CD45 at 4 weeks after cell transplantation. Transplanted MSCs could survive in infarcted myocardium. Left ventricular ejection fraction and left ventricular fractional shortening of the MSCs transplantation group were higher significantly than those of the control group (P < 0.01). Left ventricular end-systolic diameter and end-diastolic diameter were lower significantly in the MSCs transplantation group than the control group (P < 0.01). Myocardial fibrosis and myocardial infarct size were lower significantly in the MSCs transplantation group than the control group (P < 0.01). Capillary density was increased significantly in the MSCs transplantation group compared with the control group (P< 0.01). Peripheral blood MSCs transplantation increases new capillary density in infarcted myocardium and improves cardiac function.

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