Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (27): 5035-5038.doi: 10.3969/j.issn.1673-8225.2011.27.021

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Immunosuppression of allogeneic bone marrow-derived mesenchymal stem cells-induced osteoblasts on T cells in mixed lymphocyte culture system

Pan Rong-gui1, Fan Qie1, Deng Yao-liang2, Zhao Jin-min1   

  1. 1Department of Orthopaedic Trauma and Hand Surgery, 2Department of Urinary Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning  53002, Guangxi Zhuang Autonomous Region, China
  • Received:2011-01-04 Revised:2011-03-21 Online:2011-07-02 Published:2011-07-02
  • Contact: Zhao Jin-min, Professor, Doctor, Doctoral supervisor, Department of Orthopaedic Trauma and Hand Surgery, First Affiliated Hospital of Guangxi Medical University ,Nanning 53002, Guangxi Zhuang Autonomous Region, China zhaojinmin@126.com
  • About author:Pan Rong-gui☆, Studying for doctorate, Attending physician, Department of Orthopaedic Trauma and Hand Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 53002, Guangxi Zhuang Autonomous Region, China zhaojinmin@126.com
  • Supported by:

    a grant from Hospital Foundation Program*

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Abstract:

BACKGROUND: Results of immunclogical response of allogeneic bone marrow-derived mesenchymal stem cells-induced osteoblast transplantation are varied.
OBJECTIVE: To investigate the features of immune adjustment function derived from the interaction of osteoblasts derived from mesenchymal stem cells (ODCs) with T cells in vitro.
METHODS: Rabbit bone marrow mononuclear cells were isolated by Ficoll-Hypaque density gradient, ex vivo culture-expanded, and obtained after the third passage. The classic chemical induced ODCs at different proportion were added to T cells to form two-way mixed lymphocyte culture system. At 3, 5, 7 days, MTT assay was used to detect T cell proliferation in each group. T-cell subsets apoptosis was analyzed using flow cytometric analysis after 24 hours.
RESULTS AND CONCLUSION: ODCs inhibited T cell proliferation in a dose-effect manner in a certain range. With increasing ODC dose and time, inhibition was enhanced. Comparison of mean inhibition rate of three times showed that (1: 20) group was less than (1: 80) group, and (1: 40) group was less than (1: 80) group (P < 0. 05). ODCs can cause apoptosis of T cell subsets, including CD4+ (apoptosis rate 8.57%) subtypes less than CD8+ (apoptosis 15.31%; P < 0.01). Results showed that ODCs in vitro can suppress T cells proliferation through pathway of cell apoptosis, especially for CD8+. However, this inhibition is not particularly strong, showing that the ODCs have some degree of immunity, but it is very low.

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