Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (27): 5026-5030.doi: 10.3969/j.issn.1673-8225.2011.27.019

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Influence of tail vein administration of bone marrow mesenchymal stem cells on expression of brain-derived neurotrophic factor and nerve growth factor after spinal cord injury in rats

Dong Feng, Lin Jian-hua, Wu Zhao-yang   

  1. Department of Orthopedics, First Affiliated Hospital of Fujian Medical University, Fuzhou  350005, Fujian Province, China
  • Received:2011-01-12 Revised:2011-03-16 Online:2011-07-02 Published:2011-07-02
  • About author:Dong Feng★, Master, Attending physician, Department of Orthopedics, First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, Fujian Province, China dongfengok555@sina.com
  • Supported by:

    the Major Project of Fujian Science and Technology Bureau, No. 2002Y014*; the Project of Fuzhou Science and Technology Bureau, No. 2002-14*

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Abstract:

BACKGROUND: Transplantation of bone marrow mesenchymal stem cells has an effect on the treatment of spinal cord injury, but the mechanism is not fully understood.
OBJECTIVE: To explore the influence of intravenous administration of bone marrow mesenchymal stem cells on expression of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) after spinal cord injury in rats and to study possible mechanism of intravenous administration of bone marrow mesenchymal stem cells after spinal cord injury in adult rats.
METHODS: Modified Allen method was applied to induce T10 spinal cord injury to prepare rat models of paraplegia. There were 6 rats in sham operation group and 24 rats in injury group. Then, the injury group was randomly divided into transplantation group and control group. Rats in the sham operation and transplantation groups received tail vein transplantation of 1 mL bone marrow mesenchymal stem cells (1×106 cells), while those in the control group were treated with PBS.
RESULTS AND CONCLUSION: After spinal cord injury in rats, the expression of BDNF and NGF was improved and advanced to increase via intravenous administration of bone marrow mesenchymal stem cells. Maybe it is one of the mechanisms of promoting restoration of injured spinal cord and improving motor function.

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