Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (27): 5016-5020.doi: 10.3969/j.issn.1673-8225.2011.27.017

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Experimental study and short-term clinical follow-up of bone mesenchymal stem cells transplantation for repair of spinal cord injury  

Gao De-xuan1, Jia Quan-zhang2, Li Dong-jun1, Chen Yu-bing3, Sun Jing-hai2, Wang Feng-hua2, Xu Shuang2, Liu Li-ping3, Jiang Da-wei1   

  1. 1Medical School of Yanbian University, Yanji  133002, Jilin Province, China; 2The 208 Hospital of Chinese PLA, Changchun  130062, Jilin Province, China; 3Second Affiliated Hospital of Jilin University, Changchun  130000, Jilin Province, China
  • Received:2011-02-09 Revised:2011-04-09 Online:2011-07-02 Published:2011-07-02
  • Contact: Jia Quan-zhang, Doctor, Chief physician, the 208 Hospital of Chinese PLA, Changchun 130062, Jilin Province, China jqz208@162.com
  • About author:Gao De-xuan★, Studying for master’s degree, Medical School of Yanbian University, Yanji 133002, Jilin Province, China gdx2012@126.com
  • Supported by:

    Jilin Science and Technology Development Plan, No.20080446*

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Abstract:

BACKGROUND: Studies of bone marrow mesenchymal stem cells (BMSCs) have gradually experienced a transition from animal experiments to clinical application. However, the mechanism is still unclear.
OBJECTIVE: To observe the repairing effect and short-term effect of BMSCs transplantation on rats motor function.
METHODS: Spinal cord injury models were prepared by modified Allen’s crack method; the BMSCs which were isolated and cultured in vitro were transplanted into the model through the vena caudalis and the local lesion. The behavioral changes were observed using the modified Tarlov score standard and the pathological changes under the light microscope. Five patients with spinal cord injury were transplanted autologous BMSCs via in suit injection, lumbar puncture and venous injection. Neurologic function and ability of daily living were evaluated.
RESULTS AND CONCLUSION: After 15 days, there was a significant increase in the score of motion function of rats receiving BMSCs transplantation as compared with the control group. Through observing the spinal pathological section after transplantation, there was significantly more recovery in the BMSCs group than the model group at post-transplantation 7, 15, 30 days. After 6 months of short-term observation, the neurologic function and ability of daily living were improved in patients undergoing BMSCs transplantation.

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