Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (27): 4998-5001.doi: 10.3969/j.issn.1673-8225.2011.27.013

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Pyrroloquinoline quinone and proliferation of neural stem cells

Xiong Shun-hua1, Li Qing2, Yao Bai-chun3, Zhao Dan1   

  1. 1Department of Physiology, Hubei University of Medicine, Shiyan  442000, Hubei Province, China
    2Department of Anesthesiology, Taihe Hospital Affiliated to Hubei University of Medicine, Shiyan  442000, Hubei Province, China
    3Department of Anatomy, Hubei University of Medicine, Shiyan  442000, Hubei Province, China
  • Received:2011-01-05 Revised:2011-04-11 Online:2011-07-02 Published:2011-07-02
  • About author:Xiong Shun-hua★, Master, Associate professor, Department of Physiology, Hubei University of Medicine, Shiyan 442000, Hubei Province, China xiongshunhua@hotmail.com
  • Supported by:

    the Science and Technology Innovation Team of Excellent Middle-Aged People and Youth of Yunyang Medical College, No. 2005CX01*

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Abstract:

BACKGROUND: At present, scholars mainly used growth factor-like substance to promote the division and proliferation of neural stem cells. We assumed that pyrroloquinoline quinone (PQQ) micromolecular compound can accelerate the division and proliferation of neural stem cells.
OBJECTIVE: To investigate the promoting effects of PQQ on the proliferation of rat adult neural stem cells in vitro. 
METHODS: The neural stem cells of Wistar rat were cultured in vitro, and 1×10-8 mol/L PQQ was administered into the culture fluid. An equal volume of Hanks solution was added in the control group. The cell cycle was examined by the flow cytometry. The cyclin-dependent Kinase (CDK2) and cyclin-dependent kinase inhibitor (P27) expressions were detected by immunohistochemical staining and western blot assay.
RESULTS AND CONCLUSION: Compared with the control group, after 16 and 24 hours of PQQ administered into the culture fluid, the rate of cells in S and G2 stage was increased and the apoptosis rate of cells was decreased markedly. The expression of CDK2 protein was noticeably increased, and the expression of P27 protein was decreased. Results suggest that low dose of PQQ could encourage the proliferation of the neural stem cells of rats.

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