Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (27): 4988-4992.doi: 10.3969/j.issn.1673-8225.2011.27.011

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Biocompatibility of procine adipose derived stem cells and acellular dermal matrix

Zhao Bao-cheng1, Wang Zhen-jun1, Han Jia-gang1, Gu Bei2, Xu Hui-min3, Yu Xue-qiao1   

  1. 1Department of General Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing  100020, China
    2Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing  100730, China
    3Department of General Surgery, Weifang People’s Hospital, Weifang  261041, Shandong Province, China
  • Received:2011-01-10 Revised:2011-04-22 Online:2011-07-02 Published:2011-07-02
  • Contact: Wang Zhen-jun, Doctor, Chief physician, Department of General Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China wang3zj@sohu.com
  • About author:Zhao Bao-cheng☆, Studying for doctorate, Department of General Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China Zhaobc2010@163.com
  • Supported by:

    Program for Outstanding Medical Field Leaders, Beijing, No. 2009-1-03*; the New Century National Hundred, Thousand and Ten Thousand Talent Project, No. 08-009*

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Abstract:

BACKGROUND: Acellular dermal matrix has been widely used as a repair material in organ and tissue repair.
OBJECTIVE: To investigate the biocompatibility of procine adipose derived stem cells and acellular dermal matrix.
METHODS: Porcine adipose derived stem cells were obtained and cultured with collagenase digestion method. Flow cytometric analysis and adipogenic, octeogenic differentiation analysis were used to identify the stem cells.
RESULTS AND CONCLUSION: The stem cells isolated from subcutaneous adipose tissue were positive for CD44 and CD105, negative for CD34 and CD45. After cultured in mesenchymal stem cell adipogenic and octeogenic differentiation basal medium respectively for 3 weeks, the cells were positive for oil red O staining and alizarin bordeaux staining. Both hematoxylin-eosin staining and scanning electron microscopy analyses confirmed that adipose derived stem cells could adhere to acellular dermal matrix. Adipose derived stem cells have good biocompatibility with acellular dermal matrix. Adipose derived stem cells-seeded acellular dermal matrix can be used in tissue engineering as a mechanical reconstructive agent and as a biolayer of stem cells for targeted delivery.

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