Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (27): 4984-4987.doi: 10.3969/j.issn.1673-8225.2011.27.010

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Isolation, culture and osteogenic differentiation of human adipose-derived stem cells 

Bai Wen1, Yin Shuo2, Cui Lei2, Jiang Ming1   

  1. 1Department of Hematology, First Affiliated Hospital of Xinjiang Medical University, Xinjiang Hematology Institute, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
    2Shanghai Tissue Engineering Research and Development Center, Shanghai  200030, China
  • Received:2011-02-26 Revised:2011-06-03 Online:2011-07-02 Published:2011-07-02
  • Contact: Cui Lei, Doctor, Professor, Shanghai Tissue Engineering Research and Development Center, Shanghai 200030, China Correspondence to: Jiang Ming, Master, Chief physician, Department of Hematology, First Affiliated Hospital of Xinjiang Medical University, Xinjiang Hematology Institute, Urumqi 830054, Xinjiang Uygur Autonomous Region, China jiangmingyy@yahoo.com.cn
  • About author:Bai Wen★, Master, Physician, Department of Hematology, First Affiliated Hospital of Xinjiang Medical University, Xinjiang Hematology Institute, Urumqi 830054, Xinjiang Uygur Autonomous Region, China baiwenbox@yahoo.cn
  • Supported by:

    the Graduate Student Research Fund*

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Abstract:

BACKGROUND: Adipose-derived stem cells (ADSCs) can secrete a large number of immune regulatory factors, not cause T-cell cytotoxicity, and adjust the type and quantity of T lymphocytes.
OBJECTIVE: To establish a method for the isolation and culture of human ADSCs and to explore the ability of osteogenic differentiation.
METHODS: ADSCs were isolated from the inguinal fat pads and digested with 0.1% type I collagenase, and cultured in osteogenic medium to study the osteogenic differentiation potential by using alkaline phosphatase staining, alizarin red staining and RT-PCR.
RESULTS AND CONCLUSION: ADSCs could be expanded steadily in vitro and flow cytometry revealed high expression of stem cell-associated markers. The cytoplasm of the induced cells became black by alkaline phosphatase staining, the mineralized nodule became reddish-brown by alizarin red, RT-PCR detection of alkaline phosphatase positive expression. Human ADSCs are an ideal source of adult stem cells for use in the organization repair and cell therapy.

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