Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (27): 4979-4983.doi: 10.3969/j.issn.1673-8225.2011.27.009

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Multiple factors-induced differentiation of rat adipose tissue-derived stem cells into neuron-like cells

Zhang Li-hua1, 2, Tang Yin-juan2, 3, Dong Wei-ren2, Guo Jia-song2, Chen Xiao-yan1, Wang Hai-hong2, Chen Pan-pan2, Ye Quan-ying1, Feng Shu-lan1, Zhang Yi2   

  1. 1Department of Histology and Embryology, Medical College of Foshan University, Foshan 528000, Guangdong Province, China
    2Department of Histology and Embryology, Basic Medical College of Southern Medical University, Guangzhou  510515, Guangdong Province, China
    3Department of Histology and Embryology, Xiangnan University, Chenzhou  418000, Hunan Province, China
  • Received:2011-02-25 Revised:2011-05-27 Online:2011-07-02 Published:2011-07-02
  • Contact: Dong Wei-ren, Doctor, Professor, Department of Histology and Embryology, Basic Medical College of Southern Medical University, Guangzhou 510515, Guangdong Province, China wrdong@163.com
  • About author:Zhang Li-hua★, Master, Experimentalist, Department of Histology and Embryology, Medical College of Foshan University, Foshan 528000, Guangdong Province, China; Department of Histology and Embryology, Basic Medical College of Southern Medical University, Guangzhou 510515, Guangdong Province, China zlhfs@163.com Tang Yin-juan, Master, Lecturer, Department of Histology and Embryology, Basic Medical College of Southern Medical University, Guangzhou 510515, Guangdong Province, China; Department of Histology and Embryology, Xiangnan University, Chenzhou 418000, Hunan Province, China Vigor1980@126.com Zhang Li-hua and Tang Yin-juan equally contributed to this paper.
  • Supported by:

    the Scientific Research Program of Foshan Health Bureau, No. 2010177*; the Science and Technology Development Program of Medicine, Foshan Science and Technology Bureau, No. 201008063*; the Young and Middle-aged Talent of Guangdong High Institute, No. C1010086*; the Natural Science Foundation of Guangdong Province, No. 8151051501000 005*; the Scientific Research Program of Foshan University, No. 2009*; General Scientific Research Program of Xiangnan University, No. 2009Y029*

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Abstract:

BACKGROUND: Differentiation of adipose tissue-derived stem cells (ADSCs) into neurons has been reported. However, little data are available regarding differential adhesion-purified ADSCs differentiation into neuron-like cells induced by multiple factors.
OBJECTIVE: To explore the feasibility of inducing rat ADSCs into neuron-like cells with a new scheme and medium containing multiple factors.
METHODS: ADSCs were isolated from the fat pads in groins and epididymis of male Sprague Dawley rats by enzyme digestion, and purified by differential attachment method. Fluorescent immunocytochemistry was used to identify the phenotype of the cells (CD44, CD49d and CD106 expressions). Differentiation of ADSCs into neuron-like cells was induced in cocktail-like neuronal differential medium containing insulin, hydrocortisone, valproic acid and forskolin. Also, fluorescent immunocytochemistry was used to identify the markers of the differentiated cells (Nestin and NeuN).
RESULTS AND CONCLUSION: After sequentially isolated, purified and cultured, the obtained cells presented with long-fusiform shape, with clear nucleoli and light stained cytoplasm. They were positive for CD44 and CD49d, but negative for CD106. The application of NIM resulted in morphological changes of ADSCs during the first 6 hours, shown by pyramidal, irregular or triangular cell body with short, thin finger-like cell processes. After 24 hours of induction, most of the cells developed multiple branched processes resembling those of neurons and positive for Nestin and NeuN. Results showed that ADSCs could be induced to differentiate into neuron-like cells in cocktail-like neuronal differential medium.

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