Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (27): 4955-4958.doi: 10.3969/j.issn.1673-8225.2011.27.004

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Differentiation ability of bone marrow mesenchymal stem cells cultured by density gradient centrifugation in vitro

Mu Xiao-hong1, Zhao Zi-yi2, Xu Lin2, Wang Shuo-ren3, Zhu Ling-qun3, Liu Tong-hua1   

  1. 1Beijing University of Chinese Medicine, Beijing  100029, China; 2Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing  100700, China; 3Central Laboratory,  Beijing University of Chinese Medicine, Beijing  100700, China
  • Received:2011-03-19 Revised:2011-05-31 Online:2011-07-02 Published:2011-07-02
  • Contact: Liu Tong-hua, Doctor, Doctoral supervisor, Chief physician, Professor, Beijing University of Chinese Medicine, Beijing 100029, China thliu@tom.com
  • About author:Mu Xiao-hong☆, Doctor, Associate chief physician, Beijing University of Chinese Medicine, Beijing 100029, China mxh_2004@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30600847*

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Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) are capable of multiple directional differentiation, and are potential seed cells and target cells for gene therapy.
OBJECTIVE: To observe in vitro cultured rabbit BMSCs, to understand its biological properties.
METHODS: Density gradient centrifugation cultivation was utilized to culture rabbit BMSCs. The morphology and characteristics of cultured cells were observed. The surface markers CD29, CD34, CD44, CD45, CD166 and HLA-DR of cells were identified by flow cytometry, and its biological properties were identified.
RESULTS AND CONCLUSION: High purity of rabbit BMSCs could be achieved by the density gradient centrifugation. The expressions of cell phenotypes were shown as follows respectively: CD29+, CD34-, CD44+, CD45-, CD166+, HLA-DR-. Under appropriate conditions, BMSCs were induced to differentiate into osteoblasts and cartilage cells.

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