Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4351-4354.doi: 10.3969/j.issn.1673-8225.2011.23.039

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Comparison of two blood cell separators in collection of peripheral blood stem cell/progenitor cells and effectiveness of transplantation

Ye Shu-lai1, Ying Mei-ai2, Chen Jia-ping2, Geng Liang-quan3   

  1. 1Clinical Laboratory, 2Department of Blood Transfusion, 3Department of Haematology, Anhui Provincial Hospital, Hefei  230001, Anhui Province, China
  • Received:2010-12-14 Revised:2011-01-31 Online:2011-06-04 Published:2011-06-04
  • About author:Ye Shu-lai, Associate chief technician, Clinical Laboratory, Anhui Provincial Hospital, Hefei 230001, Anhui Province, China yeshulai@yahoo.cn

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Abstract:

BACKGROUND: Peripheral blood stem cells/progenitor cells were collected in vitro using blood cell separators, which directly affects the features and quantity of collected cells, additionally, affects hematopoietic reconstitution of recipients. 
OBJECTIVE: To evaluate the efficiencies of Fenwal CS-3000 Plus and Amicus blood cell separators in collecting peripheral blood stem cells/progenitor cells and effectiveness of transplantation.
METHODS: Totally 51 cases received allogenic peripheral blood stem cells transplantation were selected, including 27 cases, mean aged (34.2±10.6) years, who performed stem cell apheresis procedures using Fenwal CS-3000 Plus blood cell separator and 24 donors, mean aged (35.4±12.1) years, using Amicus blood cell separator. The number of nucleated cells and CD34+ cells, collection efficiency, collection amount of red blood cell and the time of the hematopoieteic reconstitution of two devices were compared.
RESULTS AND CONCLUSION: There were no significant differences in the total number of nucleated cells and CD34+ cells, collection efficiency, collection amount of red blood cell and the time of the hematopoieteic reconstitution between the two devices. However, the amount of platelet in the stem/progenitor cell products of the Fenwal CS-3000Plus group was obviously greater than that of the Amicus group (P < 0.01). There is no difference in the collection efficiencies of the two blood cell separators in the peripheral blood stem cells/progenitor cell and the effectiveness of transplantation. The Amicus cell separator is better for the donors with thrombocytopenia.

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