Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4325-4329.doi: 10.3969/j.issn.1673-8225.2011.23.033

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Effect of arsenic trioxide on proliferation of T cell lymphoma cell line Hut-78

Zhang Xue-mei1, Wang Ting2, Li Xiao-jin3, Zhao Liang1, Li Hui-min1   

  1. 1Department of Hematology, the First Affiliated Hospital of Kunming Medical University, Kunming  650031, Yunnan Province, China
    2Department of Hematology, Yunnan Third People’s Hospital, Kunming  650032, Yunnan Province, China
    3Yunnan Second People’s Hospital, Kunming  650031, Yunnan Province, China
  • Received:2010-12-17 Revised:2011-03-16 Online:2011-06-04 Published:2011-06-04
  • About author:Zhang Xue-mei★, Master, Professor, Chief physician, Department of Hematology, the First Affiliated Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China zxm8955@sohu.com
  • Supported by:

    Graduate Innovation Fund of Kunming Medical University, No. KM2008L55*

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Abstract:

BACKGROUND: In recent years, although some effective therapies of arsenic trioxide for T cell lymphoma have been reported, this mechanism is yet unknown.
OBJECTIVE: To investigate the effect of arsenic trioxide on inhibiting proliferation, inducing apoptosis and cell cycle of T cell lymphoma cell line Hut-78 cells.
METHODS: The inhibitory effect of arsenic trioxide (2, 5, 10 μmol/L for 24, 48, 72 hours) on proliferation and its inducing apoptosis effect, influence on cell cycle of Hut-78 cell line were detected by MTT, flow cytometry with PI staining and terminal deoxynucleotidy transferase-mediated dUTP nick end-labeling (TUNEL) assay. The transcription level changes of bcl-2 gene and vascular endothelial growth factor (VEGF) gene was measured by RT-PCR.
RESULTS AND CONCLUSION: In the concentration range of 2-10 μm/L of arsenic trioxide, with the increasing of concentration and extending of action time, the growth inhibition of Hut-78 cells was gradually increased. The apoptosis of Hut-78 mainly occurred at G2-M phase. The effect of arsenic trioxide to induce Hut-78 apoptosis could be related to down-regulation of bcl-2 gene. Arsenic trioxide has an influence on inhibiting proliferation and inducing apoptosis of Hut-78 in a time- and dose-depended manner.

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