Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4316-4320.doi: 10.3969/j.issn.1673-8225.2011.23.031

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Immigration and differentiation of bone marrow mesenchymal stem cells transfected by CXCR-4 gene after intravenous transplantation in mice with spinal cord injury

Song Chen-yang1, Xu Hao2, Chen Jian-mei2, Pan Ting-ming3, Yao Xiao-dong2   

  1. 1Union Hospital of Fujian Medical University, Fuzhou  350001, Fujian Province, China
    2Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA, Fuzhou  350025, Fujian Province, China
    3Second Affiliated People’s Hospital, Fujian University of Traditional Chinese Medicine, Fuzhou  350003, Fujian Province, China
  • Received:2011-01-09 Revised:2011-02-12 Online:2011-06-04 Published:2011-06-04
  • Contact: Xu Hao, Chief physician, Professor, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA, Fuzhou 350025, Fujian Province, China xiuhao@medmail.com.cn
  • About author:Song Chen-yang★, Master, Physician, Union Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China charliesong86@163.com
  • Supported by:

    the Key “Eleventh-Five Year” Medical and Health Research Program of Nanjing Military Area Command of Chinese, PLA, No. 07Z037*

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Abstract:

BACKGROUND: Previous studies demonstrated that stromal cell derived factor-1/CXCR-4 axis system plays a role in mediating bone marrow mesenchymal stem cells’ (BMSCs) orient immigration.
OBJECTIVE: To investigate the feasibility of BMSCs transfected by CXCR-4 gene after intravenous transplantation for treatment of spinal cord injury (SCI).
METHODS: C57BL/6 mice T10 SCI model was constructed by spinal cord transection method, and at 7 days after modeling, which were randomly divided into 3 groups: experimental group, control group and blank group. Mice were intravenous injected by the suspensions of allogeneic BMSCs transfected by CXCR-4 gene in experimental group. Mice were respectively intravenous injected by the suspensions of non-transfected allogeneic BMSCs and non-cell culture medium in control and blank groups. At 7, 14, 21, 28 days after transplantation, the survival and differentiation of green fluorescence protein positive cells to all cells in different site after transplantation were detected by laser scanning confocal microscope and Immunohistochemical double staining method. BBB score was used to evaluate the recovery condition of neuromotor function of mice.
RESULTS AND CONCLUSION: After transplantation, green fluorescence protein positive cells significantly gathered in the injured part of spinal cord in experimental group at each time point, and the number gradually increased, and the transport factor was obviously higher than in control and blank groups. The proportion of neural cells from BMSCs in transplanted cells in experimental group was also higher than that in control and blank groups. The neuromotor function of mice recovered obviously. BMSCs transfected by CXCR-4 gene after intravenous transplantation showed stronger orient immigration ability to SCI focus, which can survive and differentiate in the SCI focus, and play a role in repairing SCI.

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