Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4312-4315.doi: 10.3969/j.issn.1673-8225.2011.23.030

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Effect of epidermal growth factor on proliferation and migration of human amniotic mesenchymal stem cells

Li Cai-hong, Shi Ping, Pang Xi-ning   

  1. Laboratory of Stem Cells and Regenerative Medicine, Key Laboratory of Cell Biology of the Ministry of Health, China Medical University, Shenyang  110001, Liaoning Province, China
  • Received:2011-01-04 Revised:2011-03-11 Online:2011-06-04 Published:2011-06-04
  • Contact: Pang Xi-ning, Doctor, Professor, Laboratory of Stem Cells and Regenerative Medicine, Key Laboratory of Cell Biology of the Ministry of Health, China Medical University, Shenyang 110001, Liaoning Province, China pxining@yahoo.com
  • About author:Li Cai-hong, Laboratory of Stem Cells and Regenerative Medicine, Key Laboratory of Cell Biology of the Ministry of Health, China Medical University, Shenyang 110001, Liaoning Province, China licaihong6812@126.com
  • Supported by:

    Shenyang Science and Technology Plan, No. 2009-090063, 2011-F10-222-4-00**

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Abstract:

BACKGROUND: Human amniotic mesenchymal stem cells (hAMSCs) have the ability to improve skin wound healing.
OBJECTIVE: To explore the effects of epidermal growth factor (EGF) on hAMSCs proliferation and migration in vitro. 
METHODS: Through isolation, identification and culture, the third generation of hAMSCs were obtained. After treatment with different concentrations of EGF, the absorbance was measure to calculate survival rate of the cells using MTT method. We also counted the number of cells through Transwell to determine the effect of EGF on cell migration. With inhibitors AG1478, LY294002 of EGFR and PI3K, we tested their influence on cell migration. 
RESULTS AND CONCLUSION: EGF caused 1.55 times increase in the absorbance, and 1.5 times increase in the number of cell across the film. So AG1478 and LY294002 had inhibitory effect on the migration hAMSCs treated with EGF. It is indicated that   the proliferation and migration ability of hAMSCs enhanced following treatment with EGF, and hAMSCs migration is related to PI3K signaling pathways.

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