Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4299-4302.doi: 10.3969/j.issn.1673-8225.2011.23.027

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Ligustrazine and traumatic brain tissue homogenate for inducing bone marrow mesenchymal stem cells differentiate into neuron-like cells

Liang Feng, Ma Jie-hua, Yang Li-na   

  1. Department of Anatomy, Hebei North University, Zhangjiakou  075000, Hebei Province, China
  • Received:2011-02-01 Revised:2011-05-16 Online:2011-06-04 Published:2011-06-04
  • Contact: Zhang Hui, Master, Professor, Department of Anatomy, Hebei North University, Zhangjiakou 075000, Hebei Province, China 274272813@qq.com
  • About author:Liang Feng★, Studying for master’s degree, Department of Anatomy, Hebei North University, Zhangjiakou 075000, Hebei Province, China fliangbadboy@sina.com
  • Supported by:

    a grant by Hebei Provincial Education Department, No. 2007304*

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Abstract:

BACKGROUND: Both ligustrazine and traumatic brain tissue homogenate could induce bone marrow mesenchymal stem cells (BMSCs) differentiate to neuron-like cells.
OBJECTIVE: To investigate the combined effects of ligustrazine and traumatic brain tissue homogenate for inducing BMSCs differentiate into neuron-like cells.
METHODS: BMSCs isolated from Wistar rats were randomly divided into four groups: blank control, ligustrazine induction, traumatic brain homogenate induction, and combined induction. The morphology of the cells were observed under inverted microscope, and the differentiation rate of cells at different time points was statistically analyzed. Some cells were determined with neuronspecific enolase, glial fibrillary acidic protein immunocytochemistry and immunofluorescence staining.
RESULTS AND CONCLUSION: The ligustrazine combined with traumatic brain tissue homogenate can induce BMSCs differentiate into neuron-like cells, the induced differentiation rate increased as the inducting time, so the combination has an important application value. Neuronspecific enolase and glial fibrillary acidic protein are of great significance in the differentiation signals.

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