Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4287-4290.doi: 10.3969/j.issn.1673-8225.2011.23.024

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Effect of four traditional Chinese medicine decoctions and ethanolic extract from Saussurea involucrata on bone marrow mesenchymal stem cell proliferation activity in vitro

Zhang Ai-guo1, Cai Jian-ping1, Xu Bao-man1, Tan Xiang-ling2   

  1. 1Department of Orthopaedics, Wuxi Hospital of TCM, Nanjing University of Chinese Medicine, Wuxi  214001, Jiangsu Province, China
    2Life School of Nantong University, Nantong  216001, Jiangsu Province, China
  • Received:2010-12-11 Revised:2011-02-14 Online:2011-06-04 Published:2011-06-04
  • Contact: Cai Jian-ping, Master, Chief physician, Department of Orthopaedics, Wuxi Hospital of TCM, Nanjing University of Chinese Medicine, Wuxi 214001, Jiangsu Province, China hazag@163.com
  • About author:Zhang Ai-guo☆, Doctor, Associate chief physician, Master’s supervisor, Department of Orthopaedics, Wuxi Hospital of TCM, Nanjing University of Chinese Medicine, Wuxi 214001, Jiangsu Province, China hazag@163.com
  • Supported by:

    Specific Fund for Traditional Chinese Medicine Research, State Administration of Traditional Chinese Medicine of China, No. 06-073921*

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Abstract:

BACKGROUND: Recent medical literature reports indicate Chinese medicine can promote bone mesenchymal stem cells (BMSCs) proliferation.
OBJECTIVE: To explore the effect of single herb Radix Astragali, Salvia, Herba Epimedii, and compound Youguiyin decoction and ethanolic extract of Saussurea involucrata on rat BMSCs proliferative activity in vitro.
METHODS: BMSCs were isolated and cultured from rat femur and humerus by conventional method. Through high pressure sterilization, the ethanolic extract of Saussurea involucrata and other single herb and compound herb decoction were administered to the third passage BMSCs with 10-2, 10-3, 10-4, 10-5 concentrations. The cell proliferative activity was determined by MTT method 3 days after culture.
RESULTS AND CONCLUSION: The herb decoction of Radix Astragali, Youguiyin and the ethanolic extract of Saussurea involucrata promoted BMSCs proliferation with all four concentrations (P < 0.01). The ethanolic extract of Saussurea involucrata and the herb decoction of Radix Astragali and Youguiyin showed the positive effect on rat BMSCs proliferative activity in vitro.

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