Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4282-4286.doi: 10.3969/j.issn.1673-8225.2011.23.023

Previous Articles     Next Articles

Effect of tenuigenin on the Caspase-3 and Par-4 expression of neural stem cells induced by beta-amyloid protein

Zhang Xiao-mei, Sun Guang-tao, Huang Zuo-yi, Wu Cheng-ji, Qi Xun-zhong, Zhu Xiao-feng   

  1. Institute of Neuroscience, Jiamusi University, Jiamusi  154007, Heilongjiang Province, China
  • Received:2010-12-28 Revised:2011-02-25 Online:2011-06-04 Published:2011-06-04
  • Contact: Zhu Xiao-feng, Doctor, Professor, Chief physician, Doctoral supervisor, Institute of Neuroscience, Jiamusi University, Jiamusi 154007, Heilongjiang Province, China sgtlunwen@163.com
  • About author:Zhang Xiao-mei★, Master, Professor, Chief physician, Master’s supervisor, Institute of Neuroscience, Jiamusi University, Jiamusi 154007, Heilongjiang Province, China sgtlunwen@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30971039*; the Natural Science Foundation of Heilongjiang Province, No. D2007-02*; the Natural Science Foundation of Heilongjiang Province, No. D200617*

PDF

354

Abstract:

BACKGROUND: The effect of tenuigenin which has a good nerve protection to neural stem cells has not been reported.
OBJECTIVE: To investigate the protective effect and mechanism of tenuigenin for neural stem cells in the hippocampus impaired by β-amyloid protein.
METHODS: The third passage neural stem cells generated from the hippocampi of Kunming mice which injured by β-amyloid protein in vitro were incubated with different concentrations of tenuigenin.
RESULTS AND CONCLUSION: Immunocytochemical technique was used to detect Caspase3-positive neural stem cells. The study revealed that the expression of Par-4 and caspase-3 positive neural stem cells in the tenuigenin group were significantly lower than that in the control group with statistical significance (P < 0.05). Tenuigenin can reduce the expression of Par-4 and Caspase-3 in neural stem cells impaired by β-amyloid protein.

CLC Number: