Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4277-4281.doi: 10.3969/j.issn.1673-8225.2011.23.022

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Influence of lycium barbarum polysaccharides on proliferation of spermatogonial stem cells in vitro

Ma Liang-hong1, Qiu Zhi-jun2, Yan Sheng-nan2, Feng Li-xin3, Wang Yan-rong4, Li Pei-jun1, Chen Fu-bao1   

  1. 1Department of Urology, Affiliated Hospital of Ningxia Medical University, Yinchuan  750004, Ningxia Hui Autonomous Region, China
    2Ningxia Medical University, Yinchuan  750004, Ningxia Hui Autonomous Region, China
    3Laboratory for Germline Stem Cell Research, Shanghai Jiao Tong University School of Medicine, Shanghai  200025, China
    4Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education in Ningxia Medical University, Yinchuan  750004, Ningxia Hui Autonomous Region, China
  • Received:2011-03-22 Revised:2011-05-15 Online:2011-06-04 Published:2011-06-04
  • Contact: Chen Fu-bao, Professor, Department of Urology, Affiliated Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China
  • About author:Ma Liang-hong☆, Doctor, Associate chief physician, Department of Urology, Affiliated Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China mmm1770@163.com Ma Liang-hong and Qiu Zhi-jun contributed equally to this paper.
  • Supported by:

    the National Natural Science Foundation of China, No.30860282*; the Natural Science Foundation of Ningxia, No. NZ08107*

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Abstract:

BACKGROUND: It has potential clinical value to use spermatogonial stem cell transplantation to cure infertility. In order to obtain a large number of spermatogonial stem cells for transplantation, culture system in vitro need to be established, which is still an urgent problem at present.
OBJECTIVE: To investigate the effect of lycium barbarum polysaccharides (LBP) on proliferation of spermatogonial stem cells in vitro.
METHODS: Sertoli cells and spermatogonial stem cells were separated from testis of 4-6 days postpartum C57BL/6 male mice by two-step enzyme digestion method. The spermatogonial stem cells were seeded on the sertoli cells feeder layer and cocultured in vitro with LBP or cytokines in cell culture medium. After one-week coculture, the cell cycle and cell activity rate of spermatogonial stem cells were determined by flow cytometry. Positive rate of spermatogonial stem cells for GFRa-1, Thy-1, c-kit as cell markers were detected.
RESULTS AND CONCLUSION: Adding LBP into the culture medium alone, the amount of spermatogonial stem cell was increased apparently, and spermatogonial stem cells proliferated significantly. Adding LBP, glial cell line-derived neurotrophic factor and leukemia inhibitory factor into the culture medium together, spermatogonial stem cells proliferated more significantly (P < 0.05). Also spermatogonial stem cells after 1-week in vitro culture maintained the feature of spermatogonial stem cells within the testicular tissue, most of them remained undifferentiated state. Results indicate that LBP or combination of some cytokines can promote the proliferation of spermatogonial stem cells in vitro.

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