Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4267-4271.doi: 10.3969/j.issn.1673-8225.2011.23.020

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Surgical operation combined with autologous bone marrow stem cell mobilization to treat ischemic cerebrovascular diseases in rats

Zhang Sheng-xu, Bu Xing-yao, Cheng Pei-xun, Liu Meng, Jiang Jin-dou, Li Zhi-ying, Zhang Feng   

  1. Henan Provincial People’s Hospital, Zhengzhou  450003, Henan Province, China
  • Received:2010-12-01 Revised:2011-02-11 Online:2011-06-04 Published:2011-06-04
  • Contact: Bu Xing-yao, Doctor, Chief physician, Professor, Master’s supervisor Doctoral supervisor, Henan Provincial People’s Hospital, Zhengzhou 450003, Henan Province, China buxingyao@126.com
  • About author:Zhang Sheng-xu★, Master, Physician, Henan Provincial People’s Hospital, Zhengzhou 450003, Henan Province, China 2008zsx@163.com
  • Supported by:

    the Science and Technology Tackle Key Program of Henan Province, No. 0424410054*; Henan Technological Innovation Program for the Medicine and Health, No. 2004-70*

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Abstract:

BACKGROUND: Studies have shown that stem cell therapy can promote recovery of brain function, and stem cells combined with surgical intervention can be more effective in improving the prognosis.
OBJECTIVE: To explore the effect and mechanism of surgical operation combined with autologous bone marrow stem cell mobilization in treatment of cerebral ischemia.
METHODS: Male Sprague-Dawley rats were used to produce rat cerebral ischemia models using suture method and then divided into four groups. We used surgery, recombinant human granulocyte colony stimulating factor (rhG-CSF) and rhGM-CSF mobilization of autologous bone marrow stem cell therapy respectively.
RESULTS AND CONCLUSION: In the surgery combined with autologous bone marrow stem cell mobilization group, the neurological deficit score, cerebral infarction area and the number of apoptosis were significantly lower than those in the control group, surgery group and bone marrow stem cell mobilization group (P < 0.01); Histopathology examination showed that BrdU, BDNF positive cells and angiogenesis were much more than those in the other three groups (P < 0.05). The surgery combined with autologous bone marrow stem cell mobilization can reduce the size of cerebral infarction, inhibit cell apoptosis, promote nerve regeneration and repair, and then improve brain function.

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