Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4262-4266.doi: 10.3969/j.issn.1673-8225.2011.23.019

Previous Articles     Next Articles

Motor and somatosensory evoked potential evaluation in rat spinal cord transection models following bone marrow mesenchymal stem cell transplantation

Li Kuan-xin1,2, Li Feng1   

  1. 1Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan  430022, Hubei Province, China
    2Department of Orthopedics, First Affiliated Hospital of Shihezi University Medical School, Shihezi  832000, Xinjian Uygur Autonomous Region, China
  • Received:2010-12-09 Revised:2011-01-21 Online:2011-06-04 Published:2011-06-04
  • Contact: Li Feng, Professor, Doctoral supervisor, Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China fengli@tjh.tjmu.edu.cn
  • About author:Li Kuan-xin☆, Studying for doctorate, Associate chief physician, Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China; Department of Orthopedics, First Affiliated Hospital of Shihezi University Medical School, Shihezi 832000, Xinjian Uygur Autonomous Region, China likuanxin@sohu.com

PDF

314

Abstract:

BACKGROUND: In clinic, cortical motor evoked potentials (MEP) and somatosensory evoked potentials (SEP) are commonly used to evaluate the injury or repair of motor and sensory pathways following spinal cord injury.
OBJECTIVE: To investigate the treatment of acute complete spinal cord injury in rats with the transplantation of bone marrow mesenchymal stem cells (BMSCs) and to monitor the change in the neurologic functions of the lower limbs using MEP and SEP.
METHODS: Fifty Wistar rats were randomized into saline group, BMSCs group, brain-derived neurotrophic factor (BDNF) group, neurotrophin 3+BMSCs group, sham-operation group. Rats in the former four groups were used to produce Allen’s models of spinal cord injury before treatment. At 4, 8 and 12 weeks after treatment, rat hind limb motor function score was evaluated, and MEP and SEP were measured 1, 3, 7, 14 days after modeling.
RESULTS AND CONCLUSION: Using the MEP, we found that the motor function of each treatment groups was recovered to some extent. There was a significant difference between treatment groups and saline group (P < 0.05). The BBB score of the hind limbs showed that the motor function of the hind limbs in treatment groups was superior to that in the saline group (P < 0.05). BMSCs modified by BDNF could be transplanted into the injured spinal cord to improve the motor function of rat lower limbs. NT-3 could improve the survival rates of BMSCs and axonal regeneration following spinal cord injury.

CLC Number: