Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4247-4250.doi: 10.3969/j.issn.1673-8225.2011.23.016

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Osteogenesis-specific miRNA expression pattern analysis in osteogenic differentiation of adipose-derived stem cells

Zhang Hao1, Kang Yan1, Ma Yuan-chen2, Zhang Zi-ji1, Huang Bao-ding1, Liao Wei-ming1   

  1. 1First Affiliated Hospital, Sun Yat-sen University, Guangzhou  510000, Guangdong Province, China
    2Guangdong Provincial People’s Hospital, Guangzhou  510000, Guangdong Province, China
  • Received:2011-03-29 Revised:2011-05-06 Online:2011-06-04 Published:2011-06-04
  • About author:Zhang Hao☆, Studying for doctorate, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510000, Guangdong Province, China zhanghao-sysu@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30872615*; Denmark  Strategy Research Foundation, No. 2101-07-0120*

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Abstract:

BACKGROUND: miRNAs have emerged as important regulators in various physiological and pathological processes of cell differentiation, and can regulate the osteogenic differentiation of adipose-derived stem cells.
OBJECTIVE: To screen the osteogenesis-specific miRNAs, and analyze the expression pattern of these miRNAs in osteogenic differentiation of adipose-derived stem cells. 
METHODS: Adipose-derived stem cells were isolated and cultured from human subcutaneous fat. The osteogenesis-specific miRNAs were screened by gene microarray technique. The relative expression of these miRNAs was analyzed on 7, 14, and 21 days by RT-PCR. The osteogenesis-specific proteins were detected on 7, 14, and 21 days by enzyme linked immunosorbent assay kit.
RESULTS AND CONCLUSION: ①The 3rd passage adipose-derived stem cells were homogeneous. Osteogenesis, adipogenesis, and chondrogenesis differentiation of adipose-derived stem cells need specific condition under an inverted microscope. ②Nine osteogenesis-specific miRNAs were picked up by gene microarray technique, five were upregulated and four were downregulated. ③On day 7 in osteogenic differentiation, miR-106a expression was upregulated 1.58 folds (P < 0.05). On day 14, nine miRNAs were upregulated. On day 21, five miRNAs were upregulated and four were downregulated. ④The concentration of osteogenesis-specific proteins such as osteocalcin, alkaline phosphatase, collagen Ⅰ and bone sialoprotein were increased on day 7, peaked on day 14 and slightly decreased on day 21. 

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