Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4237-7241.doi: 10.3969/j.issn.1673-8225.2011.23.014

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Directional differentiation of adipose tissue-derived stem cells into hepatic-like cells in vitro by procedure-induction

Liu Jian1, Li Li2, Ran Jiang-hua2, Zhang Sheng-ning2, Shao Jian-chun3   

  1. 1Kunming Medical College, Kunming  650031, Yunnan Province, China
    2Department of Hepatopancreatobiliary Surgery, Ganmei Hospital Affiliated to Kunming Medical College, Kunming  650011, Yunnan Province, China
    3Department of Clinical Laboratory, Ganmei Hospital, Kunming  650011, Yunnan Province, China
  • Received:2011-02-26 Revised:2011-05-09 Online:2011-06-04 Published:2011-06-04
  • Contact: Li Li, Professor, Chief physician, Department of Hepatopancreatobiliary Surgery, Ganmei Hospital Affiliated to Kunming Medical College, Kunming 650011, Yunnan Province, China ynkmlili@yahoo.com
  • About author:Liu Jian☆, Studying for doctorate, Attending physician, Kunming Medical College, Kunming 650031, Yunnan Province, China liujianwxc1219@163.com
  • Supported by:

    the Scientific Research Foundation of Department of Science and Technology of Yunnan Province, No. 2007CA007*

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Abstract:

BACKGROUND: How to establish effective stable hepatic cell differentiation schedule, to purify or rapidly amplify stable hepatic-like cells needs to be solved before adipose tissue-derived stem cells (ADSCs) were used to treat liver diseases.
OBJECTIVE: To establish sequencing induction system of ADSCs transforming into hepatic-like cells.
METHODS: After isolated and purified the Lewis rat ADSCs, the surface marks of ADSCs were identified by flow cytometry. The rat ADSCs were transformed into the hepatic-like cells in the procedure-culture system by tris-step including hepatocyte growth factor (FGF), fibroblast growth factor-4, acid fibroblast growth factor and oncostatin M cytokine.
RESULTS AND CONCLUSION: After rat ADSCs were induced at 7, 14 and 21 days, the expression of albumin (ALB), alpha fetoprotein (AFP) and CK18 mRNA was determined and fortified over time. Hepatic-like cells had ALB synthesis function. Metabolism of ammonia and urea synthesis occurred and lasted after 9-12 days. Results suggest ADSCs were successfully differentiated into hepatic-like cells in vitro after procedure-induction.

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