Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4233-4236.doi: 10.3969/j.issn.1673-8225.2011.23.013

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Preparation and biological characteristics of feeder cells for human embryonic stem cells

Hu Jia-bo, Ma Quan-hui, Hu San-qiang, Zhou Zhong-wei, Wang Xiao-hui, Qian Hui, Xu Wen-rong   

  1. School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang  212001, Jiangsu Province, China
  • Received:2010-11-30 Revised:2011-03-16 Online:2011-06-04 Published:2011-06-04
  • Contact: Xu Wen-rong, Doctor, Professor, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212001, Jiangsu Province, China icls@ujs.edu.cn
  • About author:Hu Jia-bo☆, Doctor, Associate professor, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212001, Jiangsu Province, China hu@ujs.edu.cn
  • Supported by:

    Startup Foundation for Advanced Talents, Jiangsu University, No. 09JDG037*; the National Natural Science Foundation of China, No.31071421*

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Abstract:

BACKGROUND: It is important to establish and save a large number of high-activity feeder cells for the culture of human embryonic stem cells (hESCs).
OBJECTIVE: To separate, culture, freeze and thaw embryonic fibroblasts of ICR mice in vitro, and to explore the biological characteristics of mouse embryonic fibroblast.
METHODS: ICR mouse fetuses of 13.5 days were chosen to isolate mouse embryonic fibroblasts by trypsin stepwise. We observed the cell morphology, growth curves, cells adhesive rate, and cytochemistry staining of mouse embryonic fibroblasts after cryopreservation. The effect of mouse embryonic fibroblasts was assessed by the ability to support the growth of hESCs.
RESULTS AND CONCLUSION: Thirty minutes after passage, the adhesive rate of mouse embryonic fibroblasts was above 80%. Mouse embryonic fibroblasts proliferated quickly. AKP, POX and PAS cytochemistry staining of mouse embryonic fibroblasts were all negative, indicating that it could support hESCs in a long period. The thawed mouse embryonic fibroblasts have high biological activity, and can be used as the stable and high quality feeder cells for hESCs culture.

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