Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4229-4232.doi: 10.3969/j.issn.1673-8225.2011.23.012

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Vitamin C promotes differentiation of mouse pluripotent stem cells into cardiomyocytes in vitro

Yang Liang, Zhang Xiao-gang, Wei Xin-wei   

  1. Department of Cardiology, First Affiliated Hospital of Chongqing Medical University, Chongqing  400016, China
  • Received:2010-12-06 Revised:2011-04-22 Online:2011-06-04 Published:2011-06-04
  • Contact: Zhang Xiao-gang, Doctor, Professor, Master’s supervisor, Department of Cardiology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China zxg0233@sina.com
  • About author:Yang Liang★, Studying for master’s degree, Physician, Department of Cardiology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China yangliang428@163.com
  • Supported by:

    the Science and Technology Research Major Program of Chongqing Health Bureau, No. 20090151*

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Abstract:

BACKGROUND: Inducing pluripotent stem cells have been considered promising treatment for ischemic heart disease. However, an ideal inducing method has not been found yet.
OBJECTIVE: To investigate the effect of vitamin C on the cardiomyocytes differentiation from mouse induced pluriptent stem cells (miPS).
METHODS: miPS were recovered, passaged and form embryoid bodies. The embryoid bodies were induced by differentiation medium containing various concentration of vitamin C (10-3, 10-4, 10-5, 10-6 mol/L). Control group was not treated by inducer. The number of beating embryoid bodies were calculated.
RESULTS AND CONCLUSION: The best concentration of vitamin C to promote the cardiac differentiation of miPS was
10-4 mol/L. Beating area was found in 57.00%±3.20% of embryoid bodies, significantly greater than control group (5.13%±0.55%; P < 0.01). Special cardiac proteins α-actin and cTnT were detected. Results showed that vitamin C could redound differentiation of miPS into cardiomyocytes.

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