Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4225-4228.doi: 10.3969/j.issn.1673-8225.2011.23.011

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Biological characteristics of rat placenta-derived mesenchymal stem cells

Liu Zhi-peng1, Han Zhi-bo2,3, Meng Lei2,3, Yang Ping2,3, Zhang Rui-ting1, Shao Yuan-kang4, Wang Tao4, Li Yang-qiu1, Han Zhong-chao1,2,3   

  1. 1School of Medicine, Jinan University, Guangzhou  510632, Guangdong Province, China
    2TEDA Life Science and Technology Research Center, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin  300457, China;
    3 State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin  300020, China
    4Guangzhou Health & Biotech Co., Ltd., Guangzhou  510632, Guangdong Province, China
  • Received:2010-12-07 Revised:2011-02-01 Online:2011-06-04 Published:2011-06-04
  • Contact: Han Zhong-chao, Doctor, Doctoral supervisor, 1School of Medicine, Jinan University, Guangzhou 510632, Guangdong Province, China; 2TEDA Life Science and Technology Research Center, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300457, China; 3 State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China hanzhongchao@hotmail.com
  • About author:Liu Zhi-peng★, Studying for master’s degree, School of Medicine, Jinan University, Guangzhou 510632, Guangdong Province, China liuzhipeng7856@163.com
  • Supported by:

    Guangdong Science and Technology Department, the Cooperation Project in Industry, Education and Research of Guangdong Province and Ministry of Education of China (The Industrialization Base of Stem Cell of Guangdong Province), No. 2010B091101004*

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Abstract:

BACKGROUND: Mesenchymal stem cells (MSCs) have the potential of multilineage differentiation. Some researchers put them into tissue-regeneration or diseases treatment. There are many studies about MSCs derived from bone marrow, umbilical cord of rat or mouse. However, little is known about MSCs derived from rat placenta.
OBJECTIVE: To establish a method to isolate MSCs from rat placenta and to observe its biological characteristics in vitro.
METHODS: Rat placental MSCs (r-pl-MSCs) were separated and obtained by collagenase-digested method. Morphology of r-pl-MSCs was daily observed with inverted microscope. Growth kinetics was measured with MTT assay and growth curve was drawn. Their surface antigens and cell cycle were detected by flow cytometer. Adipogenic differentiation and osteogenic differentiation were tested by immunohistochemistry.
RESULTS AND CONCLUSION: The primary cultured cells adhered after 8 hours and formed clone within 24 hours. The size and morphology of the 4th generation cells were almost same; most cells were fusiform-shaped. Cell cycle measure showed the proportion of cells in G0/G1 phase, S phase, G2 phase was respectively 83.76%, 8.01%, 8.23%. Immunophenotype analysis showed that the cells expressed CD29 and CD90, but no CD45. Placenta-derived MSCs have potentials of adipogenic and osteogenic differentiation in special culture condition in vitro.

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