Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4215-4219.doi: 10.3969/j.issn.1673-8225.2011.23.009

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Marine phytoplankton effects on biological activity of human glioma stem cells in vitro

Duan Xiao-bing1,2, Guan Fang-xia3, Deng Xiao-hui1, Yang Bo1,2, Zhang Tian-xiang1, Tian Yi1, Qiao Xiao-jun1, Li Yuan1, Liang Shuo3, Zhu Wan-wan3   

  1. 1Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China
    2Institute of Clinical Medicine, Zhengzhou University, Zhengzhou  450052, Henan Province, China
    3Department of Bioengineering, Zhengzhou University, Zhengzhou  450001, Henan Province, China
  • Received:2010-11-30 Revised:2011-03-16 Online:2011-06-04 Published:2011-06-04
  • Contact: Yang Bo, Master, Professor, Doctoral supervisor, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China yangbo96@126.com
  • About author:Duan Xiao-bing★, Studying for master's degree, Physician, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China duanxb1983@126.com Guan Fang-xia, Doctor, Professor, Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, Henan Province, China guanfangxia@126.com Duan Xiao-bing and Guan Fang-xia contributed equally to this paper.

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Abstract:

BACKGROUND: Marine phytoplankton (MPPT) has important guiding significance for application to strengthen the research.
OBJECTIVE: To investigate the effect of MPPT on biologic activity of brain gliomastem cells (BGSCs) in vitro. 
METHODS: BGSCs cultured in the enzyme digestion way. The CD133 positive cells were sorted out by flow cytometry, and the 3rd passage of BGSCs were gathered through sub-culturing. CD133 expression was identified by flow cytometry before and after the effect of MPPT. Immunohistochemisty was used to detect Nestin and glial fibrillary acidic protein (GFAP) expression of adherent cells. The experimental group was added with different concentrations of MPPT, the negative control group was added with just PBS. 4, 6, 8, 10 g/L MPPT was added into the cell culture fluid. The cell cycle of BGSCs was inspected by flow cytometry, and inhibitory effects were detected using a microplate reader respectively at 24, 48, 72 hours later.
RESULTS AND CONCLUSION: Compared to those in the control group, BGSCs in the experimental group could not form a compact, and began to be adherent and differentiated gradually observed by an inverted microscope, with the increasing of time and concentration. CD133 expression in BGSCs after the effect of MPPT was reduced obviously. The adherent cells expressed GFAP and Nestin. The cell number in S and G2/M phase was increased, and that in G0/G1 phase was decreased. Growth curve indicated that the proliferation of BGSCs was inhibited obviously, with the increasing of time and concentration (P < 0.05-0.01). Results indicate MPTT can inhibit the proliferation of BGSCs, and promote them differentiate, which shows a dose and time dependent effect.

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