Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4207-4210.doi: 10.3969/j.issn.1673-8225.2011.23.007

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Effects of simvastatin on the apoptosis of human mesenchymal stem cells induced by high glucose and saturated fatty acids

Zhao Jie1, Li Hua-qing2, Li Jing1, Xue Guo-fang1, Chen Yan1, Qi Kai3, Dong Min3, Wang Hui-zhen3, Niu Bo4   

  1. 1The Second Hospital of Shanxi Medical University, Taiyuan  030001, Shanxi Province, China
    2The Second Hospital of Jinzhong, Jinzhong  030008, Shanxi Province, China
    3Department of Biochemistry, Shanxi Medical University, Taiyuan  030001, Shanxi Province, China
    4Department of Biotechnology, Capital Institute of Pediatrics, Beijing  100020, China
  • Received:2011-02-09 Revised:2011-03-12 Online:2011-06-04 Published:2011-06-04
  • Contact: Niu Bo, Department of Biotechnology, Capital Institute of Pediatrics, Beijing 100020, China
  • About author:Zhao Jie☆, Studying for doctorate, Associate chief physician, The Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China

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Abstract:

BACKGROUND: Previous studies demonstrated that statins can promote the proliferation and adhesion of bone marrow mesenchymal stem cells (BMSCs), inhibit apoptosis of BMSCs cultured with high glucose and saturated fatty acids.
OBJECTIVE: To investigate the effects of simvastatin on the apoptosis of human mesenchymal stem cells induced by high glucose and saturated fatty acids.
METHODS: Different concentrations of simvastatin (0.001, 0.01, 0.1, 1.0 μmol/L) was cultured with BMSCs in presence of high glucose and saturated fatty acids for 48 hours. BMSCs cultured with normal bone marrow mesenchymal stem cells induced by high glucose and saturated fatty acids were as controls. Cell morphology was observed under the inverted microscope. Effect of simvastatin on cell survival rate of BMSCs in high glucose and saturated fatty acids was measured by MTT assay. Cell apoptosis was detected by flow cytometry. LY294002 (PI3K inhibitor) was used to study the underlying mechanism of simvastatin effect on BMSCs apoptosis.
RESULTS AND CONCLUSION: Compared with induced by high glucose and saturated fatty acid group, the survival rate of BMSCs in different concentrations of simvastatin (0.001, 0.01, 0.1, 1.0 μmol/L) group was increased (P < 0.01), especially in simvastatin at concentration of 0.1μmol/L (P  < 0.01). Meanwhile, flow cytometry showed apoptosis rate was decreased in simvastatin at concentration of 0.01, 0.1, 1.0 μmol/L group (P  < 0.01), especially in 0.1 μmol/L group (P  < 0.01). The effect of simvastatin at concentration of 0.1μmol/L on BMSCs could be blocked. The results suggested that simvastatin can inhibit BMSCs apoptosis induced by high glucose and saturated fatty acids, and its mechanism may be related to PI3K/Akt pathway.

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