Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4203-4206.doi: 10.3969/j.issn.1673-8225.2011.23.006

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Induction of marrow stromal cells into myocardical cells by conditioned cultrure medium of cardiac muscle and 5-azacytidine

Wang Yang1, Shen Hui-juan2, Zhou Min-fen1, Wu Bin-qiao1, Zeng Xian-zhi2   

  1. 1Department of Clinical Medicine, College of Medicine, 2Laboratory Center of Engineering of  Machinery and Electronics, 3Department of Anatomy, College of Medicine, Jiaxing University, Jiaxing  314004, Zhejiang Province, China
  • Received:2010-12-13 Revised:2011-04-25 Online:2011-06-04 Published:2011-06-04
  • Contact: Zeng Xian-zhi, Doctor, Lecturer, Laboratory Center of Engineering of Machinery and Electronics, Jiaxing University, Jiaxing 314004, Zhejiang Province, China frankzengxzh@yahoo.cn
  • About author:Wang Yang, Department of Clinical Medicine, College of Medicine, Jiaxing University, Jiaxing 314004, Zhejiang Province, China wydc88@sina.com
  • Supported by:

    the Science and Technology Innovation Program of Undergraduate Students in Zhejiang Province, No. 2009R417002*

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Abstract:

BACKGROUND: Bone marrow stromal cells (MSCs) can significantly improve cardiac function after ischemia-induced myocardium injury, but the mechanism of differentiation of MSCs into myocardical cells and its contribution to the regeneration of cardiac function remains uncertain.
OBJECTIVE: To explore the effects of conditioned cultrure medium of cardiac muscle and 5-azacytidine on inducing MSCs into myocardial cells.
METHODS: MSCs were sorted from whole bone marrow by adherent culture. MSCs of the 6th generation were divided into 4 groups: combined group (5-azacytidine + conditioned cultrure medium of cardiac muscle; 5-azacytidine group; conditioned cultrure medium group; blank group (basal culture medium). At 3 weeks after induction by 5-azacytidine and conditioned cultrure medium of cardiac muscle, the expression of myocardial troponin was measured by immunohistochemistry and analysed by one-way analysis of variance.
RESULTS AND CONCLUSION: MSCs could be induced into myocardial cells by conditioned cultrure medium of cardiac muscle in vitro, indicating that the cytokines in the cultrure medium might play key roles in the induction. But such effect of conditioned cultrure medium of cardiac muscle was weaker than that of 5-azacytidine. The effect of combined induction was the strongest. 

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