Abstract:

BACKGROUND: Because of convenience and small injury to the body, bone marrow stromal cells (BMSCs) are the most appropriate seed cells in bone tissue engineering. But the methods in vitro cultivation and the biological characteristic are not clear. 
OBJECTIVE: To establish a simple and effective BMSCs cultured in vitro and osteoblasts induction method, and to explore the biological characteristics in vitro.
METHODS: The BMSCs of Wistar rats were seperated and purified by using of adherent method. The passage cells of the third generation were cultured in the common medium (control group) and osteogenic induction medium (experimental group) for 10 days, the growth cure of each group was drew. The alkaline phosphatase activities (APA) of two groups were detected at 4, 7, 10, 13, 16, days’ culture and cover glass was treated with Von Kossa staining.
RESULTS AND CONCLUSION: The better homogenicity of BMSCs were obtained by adherent method. The growth velocity of the cells cultured in the control group was obviously faster than that in the experimental group. The APA in control group was significantly lower than that in experimental group (P < 0.05). The Von Kossa’ staining in the experimental group was positive and negative in the control group. Adherent screening method is a simple and effective method to separate and purify BMSCs. BMSCs can be differentiated into osteoblasts in vitro.