Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (23): 4181-4184.doi: 10.3969/j.issn.1673-8225.2011.23.001

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Effect of bone marrow mesenchymal stem cells-conditioned medium on proliferation and fibrogenesis of hepatic stellate cells

Yao Zhi-cheng, Zhong Yue-si, Hu Kun-peng, Chen Si, Li Ming-liang, Fang He-ping, Deng Mei-hai   

  1. Department of Hepatobiliary Surgery, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou  510630, Guangdong Province, China
  • Received:2011-03-28 Revised:2011-04-19 Online:2011-06-04 Published:2011-06-04
  • Contact: Deng Mei-hai, Doctor, Department of Hepatobiliary Surgery, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China drdmh@medmail.com.cn
  • About author:Yao Zhi-cheng★, Studying for master’s degree, Department of Hepatobiliary Surgery, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China yzc820215@126.com
  • Supported by:

    the Natural Science Foundation of Guangdong Province, No. 8151008901000112*; the National Youth Foundation, No. 81000177*

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Abstract:

BACKGROUND: The studies concerning bone marrow mesenchymal stem cells (BMSCs) in treatment of hepatic fibrosis have obtained some outcomes. However, few studies have addressed the conditioned medium for treatment of hepatic fibrosis.
OBJECTIVE: To observe how rat BMSCs-conditioned medium to regulate the proliferation and activation of hepatic stellate cells (HSCs).
METHODS: 2 mL BMSCs conditioned medium was added in six-well plate to treat the HSCs as experimental group one. 1 mL BMSCs conditioned medium plus 1 mL fresh medium were added to treat HSCs as experimental group two. HSCs were treated with 2 mL fresh medium as the control group. Cell cycle was determined by flow cytometry, and inhibitory effect was observed. The expression of transforming growth factor (TGF)-β, tissue inhibitor of metalloproteinase-2 (TIMP-2), Collegan I mRNA in HSCs was detected by RT-PCR.
RESULTS AND CONCLUSION: After adding BMSCs conditioned medium, in group one, HSC proliferation was significantly inhibited compared with the control group, and the tendency become obvious with the prolongation of time (BMSCs conditioned medium treated 72 h > BMSCs conditioned medium treated 48 h > BMSCs conditioned medium treated 24 h, P < 0.05). The expressions of TGF-β, TIMP-2, Collegan I mRNA in HSCs were down-regulated in experimental groups one and two compared to the control group, and the tendency was followed by the increase of BMSCs conditioned medium (experimental group one > experimental group two > control group).

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