Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (19): 3472-3475.doi: 10.3969/j.issn.1673-8225.2011.19.012

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Comparison of two isolation methods for umbilical cord blood mesenchymal stem cells

Gao Yang, Li Li, Ran Jiang-hua, Chen Yi-ming, Jiang Yi-zhou   

  1. First Department of Hepatobiliary, First People’s Hospital of Kunming City, Kunming  650011, Yunnan Province, China
  • Received:2010-11-27 Revised:2011-04-18 Online:2011-05-07 Published:2011-05-07
  • Contact: Li Li, Doctor, Professor, Chief pluysician, First Department of Hepatobiliary, First People’s Hospital of Kunming City, Kunming 650204, Yunnan Province, China
  • About author:Gao Yang★, Studying for master’s degree, Physician, First Department of Hepatobiliary, First People’s Hospital of Kunming City, Kunming 650011, Yunnan Province, China
  • Supported by:

    the Major Program of Kunming Science and Technology Bureau, No. 2007CA007*

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Abstract:

BACKGROUND: There is no standard method to isolate umbilical cord blood mesenchymal stem cells (MSCs)．
OBJECTIVE: To discuss an optimized isolation method of human umbilical cord blood MSCs．
METHODS: Monocytes were isolated from umbilical cord blood using the Percoll separation medium method and hydroxyethyl starch sedimentation respectively. Cells were cultured in DMEM/F12 containing 15% newborn calf serum. The main outcome indictors were recovery rate of monocytes, generation time, cell proliferation, morphological change, and the surface markers expressed on umbilical cord blood MSCs measured by flow cytometer.
RESULTS AND CONCLUSION: Compared with the Percoll separation medium method, the recovery rate of monocytes increased and the first passage time reduced using the hydroxyethyl starch sedimentation (P < 0.01). But the morphological change and surface markers CD90, CD44, CD34 expression of two methods have no differences (P > 0.05). Therefore, the hydroxyethyl starch sedimentation has higher isolation efficiency and the shorter culture time than Percoll separation medium method. However, the quality of umbilical cord blood MSCs which are isolated by two methods has no difference.

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