Abstract:

BACKGROUND: The growth features of bone marrow stromal stem cells (BMSCs) for hypoxia ischemic encephalopathy is unclear.
OBJECTIVE: To observe growth characteristics and physical and chemical characters of BMSCs in treatment of acute anoxic-ischemia newborn rats.
METHODS: Seven-day-old rats were selected to ligate the bilateral carotid arteries to prepare ischemic models. After 2 hours, the neonatal rats deeply suffered from hypoxia (8% O2 and 92% N2) for 1-2.5 hours. The rats were divided into mild group (ischemia for 1 hour), moderate group (ischemia for 1.5 hours), severe group (ischemia for 2.5 hours), anoxia-ischemia group and sham operation group (only ligation of the left carotid artery). The growth condition of BMSCs was observed.
RESULTS: After 3-hour culture, BMSCs grew adherently in the sham operation and anoxia-ischemia groups, superior to those in the moderate and severe groups. The morphology of BMSCs recovered in the sham operation, mild and moderate groups. The apoptotic rates were similar in the sham operation and mild groups, and obviously increased in the moderate and severe groups. The activity of supernatant lactate dehydrogenase was increased in the mild and severe groups to different extent. The findings demonstrate that the effect of mild and moderate anoxia-ischemia on BMSCs is reversible, and BMSCs grow vigorously and benefit to injury repair.