Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (19): 3421-3426.doi: 10.3969/j.issn.1673-8225.2011.19.001

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A comparative study on bone marrow mesenchymal stem cells differentiated into chondrocytes induced by co-culture with chondrocytes and conditioned medium

Lü jing1, Ke Jie1, Wang Lin2, Zhang Dong-mei3, Liu Bing1, Pang Jian-liang1, Deng Tian-zheng1   

  1. 1Department of Stomatology, the General Hospital of the Air Force, Beijing  100142, China
    2Institute of Geriatric Cardiology, Chinese PLA General Hospital, Beijing  100853, China
    3Department of Stomatology, Out-patient of the Second Retired Cadres, Logistics of Beijing Military Region, Beijing  100071, China
  • Received:2010-12-06 Revised:2011-01-29 Online:2011-05-07 Published:2011-05-07
  • Contact: Deng Tian-zheng, Doctor, Attending physician, Department of Stomatology, the General Hospital of the Air Force, Beijing 100142, China dengtzh@163.com
  • About author:Lü Jing, Attending physician, Department of Stomatology, the General Hospital of the Air Force, Beijing 100142, China greatfriday@hotmail.com
  • Supported by:

     the National Natural Science Foundation of China, No.30900314*, 81000423*

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Abstract:

BACKGROUND: In vitro differentiation of bone marrow mesenchymal stem cells (BMSCs) is dependent on appropriate culture conditions to a great extent.
OBJECTIVE: To study the biological characteristics of chondrocytes differentiated from BMSCs co-cultured with chondrocytes versus cultured in conditioned medium.
METHODS: Rat BMSCs and chondrocytes were isolated and cultured in neural induction. Cellular non-contact co-culture induced method and conditioned medium induced method were taken to reduce BMSCs differentiating into chondrocytes. And the biological characteristics of the cells were evaluated by MTT, mitotic cycle, GAG secretion, RT-PCR, cell-staining methods, immunocytochemistry.
RESULTS AND CONCLUSION: The biological characters of co-culture induced cells were seemed to be better than those of conditioned medium induced cells, which point out that BMSCs co-cultured with chondrocytes are appropriate as seed cells in cartilage tissue engineering.

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