Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (18): 3319-3322.doi: 10.3969/j.issn.1673-8225.2011.18.022

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Effect of tripterygium wilfordii polyglucoside on histological changes of a rat model of chronic renal allograft rejection

Yu Peng-cheng1, Liu Yong-guang1, Li Min1, Guo Ying1, Chen Hua1, Yue Liang-sheng1, Wu Jian-ping2, Zhao Ming1   

  1. 1Organ Transplant Center, Zhujiang Hospital, Southern Medical University, Guangzhou  510280, Guangdong Province, China
    2Department of Nephrology, First Hospital of Quanzhou, Fujian Medical University, Quanzhou  362000,  Fujian Province, China
  • Received:2011-02-16 Revised:2011-03-14 Online:2011-04-30 Published:2011-04-30
  • Contact: Zhao Ming, Professor, Doctoral supervisor, Organ Transplant Center, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, Guangdong Province, China zhaoming02@hotmail.com
  • About author:Yu Peng-cheng☆, Studying for doctorate, Attending physician, Organ Transplant Center, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, Guangdong Province, China nananyu@sohu.com
  • Supported by:

    Science and Technology Plan of Quanzhou City, No. 2009Z39*

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Abstract:

BACKGROUND: Tripterygium wilfordii polyglucoside possess a variety of immune regulation. Whether it can be used for chronic renal allograft rejection needs animal experiments as well as multi-center, large-sample clinical trials.
OBJECTIVE: To explore the effect of TWP on chronic renal allograft rejection in rats.
METHODS: Orthotropic kidney transplantation was performed in strain combinations of SD-Wistar. The native kidney of the recipients were kept and used as an internal control. All recipients received a short course treatment of cyclosporine A (CsA microemulsion) (2 mg/kg/d for 10 days i.p) after transplantation to prevent acute rejection to establish chronic renal allograft rejection model. Fifteen successful recipient rats were randomized into TWP-treating group (n=8) and control group (n=7). The recipient rats of the two groups were treated with TWP at doses of 30 mg/kg (TWP-treating group) or the same volume of 0.9% saline solution (control group) per day from day 10 until week 12 after transplantation. All recipient rats were killed in week 12 after transplantation, the histology examination of grafts and native kidneys was performed in parallel according to the Banff 07 working classification for renal allograft pathology. Graft histology was quantified by using the Banff sum score. Immunohistochemistry of transforming growth factor-β1 (TGF-β1) of each allograft was examined and was semiquantitatively evaluated.
RESULTS AND CONCLUSION: All allografts of each group survived up to 12 weeks after transplantation and develop chronic renal allograft rejection, characterized by neointimal hyperplasia, interstitial fibrosis, tubular atrophy, glomerulosclerosis and mononuclear cell infiltration. Compared with those in the control group, the Banff sum scores and TGF-β1 expressions in the renal allograft tissues were significantly decreased in the TWP-treating group in week 12 ( P < 0.01 for both). The native kidney of recipients of each group showed no inflammation or histological alterations. In conclusion, TWP effectively reduces the histological damages and the expression of TGF-β1 in the renal allograft tissues and may be used as a new agent to treat chronic renal allograft rejection.

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