Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (8): 1377-1380.doi: 10.3969/j.issn.1673-8225.2011.08.011

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Histocompatibility of bone marrow mesenchymal stem cell and collagen-chitosan scaffold

Li Xiao-long1, Mu Chang-zheng2, Ma Yun-sheng2   

  1. 1Microscope Central Laboratory, 2Department of Histology and Embryology, Liaoning Medical University, Jinzhou   121000, Liaoning Province, China
  • Received:2010-09-25 Revised:2010-11-11 Online:2011-02-19 Published:2011-02-19
  • Contact: Ma Yun-sheng, Doctor, Lecturer, Department of Histology and Embryology, Liaoning Medical University, Jinzhou 121000, Liaoning Province, China
  • About author:Li Xiao-long, Experimentalist, Microscope Central Laboratory, Liaoning Medical University, Jinzhou 121000, Liaoning Province, China yunshengma@126.com
  • Supported by:

    Innovation Team of Liaoning Province Education Department, No. 2006T062*; Natural Science Foundation of Liaoning Province, No. 20072201*

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Abstract:

BACKGROUND: Recent studies have showed that the collagen-chitosan complex scaffold can be used as nerve tissue engineering scaffold, but little evidence is available in the histocompatibility study.
OBJECTIVE: To observe rabbit bone marrow mesenchymal stem cells (MSCs) growth and differentiation in the surface of collagen-chitosan complex scaffold.
METHODS: Rabbit MSCs were isolated and cultured in serum-free medium. Flow cytometry was used to analyze the immunophenotype. The MSCs were planted on the gel scaffold (experimental group) and polylysine coated coverslip (control group), the cells were cultured in neural induction medium. The cell differentiation and growth
characteristics were observed by inverted phase contrast microscope.
RESULTS AND CONCLUSION: The phenotype was CD29+, CD44+, CD166+. Inverted phase contrast microscope showed that: the MSCs grew well with the prominence process seen at 7 days in experimental group. The cells growth characteristics had no difference with the control group. The MSCs and collagen-chitosan complex scaffold have good histocompatibility.

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