Abstract:

BACKGROUND: Tissue engineering research focuses on the use of a small number of cells cultured and amplified in vitro, in certain circumstances attached to the three-dimensional porous scaffolds and in a good growth for the reconstruction and repair of tissues and organs.
OBJECTIVE: To evaluate the different concentrations of rabbit bone marrow mesenchymal stem cells (BMSCs) compounded on the chitosan scaffolds for tissue engineered regeneration and repair.
METHODS: Chitosan scaffold was prepared by freeze-drying with 5×10⁵, 95% degree of deacetylation of chitosan powder. BMSCs growth and division were observed by optical microscope, scanning electron microscopy, and MTT method with 1×10⁶, 1×10⁷, 1×108, 1×10⁹/L cells of 100 μL compound on the chitosan scaffold at 1, 3, 5, 7, 9 days.
RESULTS AND CONCLUSION: Sponge-like chitosan porous scaffold was 5 mm×5 mm×3 mm, the aperture was 190-380 μm, average aperture was 290 μm, the permeability was good, and the porosity was (84.00±4.62) %. At 72 hours after cell/scaffold co-culture, cells infiltrated the porous structure of chitosan scaffold and adhered to it. Cells of each group at concentration of 1×10⁷, 1×10⁸, 1×10⁹/L grew in cluster on the scaffold, some cells fused with the scaffold. It is indicated that 1×10⁷, 1×10⁸/L concentration of cells is more conductive to the adhesion growth of BMSCs on chitosan scaffold for tissue regeneration.