Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (4): 575-579.doi: 10.3969/j.issn.1673-8225.2011.04.002

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Toll-like receptors 4 and 9 expression in leukocytes following joint arthroplasty

Zhang Zi-ji1, Kang Yan1, Sheng Pu-yi1,2 , Zhai Qi-yi2, Zhang Hao1, Zhang Yang-chun2, Hou Chang-he2, Li Zi-qing2, Liao Wei-ming1    

  1. 1Department of Joint, First Affiliated Hospital of Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China
    2Huangpu Joint Surgery Center, First Affiliated Hospital, Sun Yat-sen University, Guangzhou  510700, Guangdong Province, China 
  • Received:2010-08-25 Revised:2010-10-16 Online:2011-01-22 Published:2011-01-22
  • Contact: Sheng Pu-yi, Associate professor, Associate chief physician, Department of Joint, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China; Huangpu Joint Surgery Center, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510700, Guangdong Province, China shengpuyi@hotmail. Com
  • About author:Zhang Zi-ji☆, Studying for doctorate, Physician, Department of Joint, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
  • Supported by:

    the International Cooperation Program of Guangdong Provincial Science and Technology Commission, No. 2006B50107004; the Science and Technology Development Program of Guangdong Provincial Science and Technology Commission, No. 2007B010600048; the Scientific Research Foundation Program of University of Tampere, Finland, No. 9J114

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Abstract:

BACKGROUND: Little researches focus on the expression of Toll-like receptors (TLRs) and its mechanism in arthroplasty patients. Evidence shows TLRs are expressed on the surface of biofilms in loosening hip prosthesis.
OBJECTIVE: To investigate the differences in TLRs 4 and 9 expression in leukocytes of patients with and without prosthesis.
METHODS: Fasting blood plasma samples were harvested 24 hours post admission and 3 days post-operation from patients undergoing arthroplasty (n=11) or arthroscopy (n=10). Cell-associated TLR 4 and 9 was measured by flow cytometry, white blood cells, C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) were tested.
RESULTS AND CONCLUSIONS: WBC, ESR, CRP were at normal level in both groups. TLR4 and 9 were mostly expressed in monocytes of peripheral blood, with low expression levels in lymphocytes and neutrophils. The expression levels of TLR 4 and TLR 9 were of no significance between post-operation and pre-operation in both groups (P > 0.05), whereas a significant change of TLR9 expression gap was found in arthroplasty patients before and after the procedure (P < 0.05). TLR 4 expression remained unchanged (P > 0.05). Results showed that in the absence of clinical infection, stress and tissue damage during arthroplasty and arthroscopy did not affect TLR4 and TLR9. TLR9 seems to be downgraded by implantation of joint prosthesis.

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