Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (3): 423-426.doi: 10.3969/j.issn.1673-8225.2011.03.011

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Allograft of microencapsulated ovarian cells affects bone collagen metabolism in ovariectomized mice

Guo Xiao-xia, Zhou Jin-ling, Xu Qing, Shi Xiao-lin   

  1. Department of Histoembryology, Capital Medical University, Beijing   100069, China
  • Received:2010-08-26 Revised:2010-10-12 Online:2011-01-15 Published:2011-01-15
  • Contact: Shi Xiao-lin, Professor, Doctoral supervisor, Department of Histoembryology, Capital Medical University, Beijing 100069, China szhyxzhx@ccmu.edu.cn
  • About author:Guo Xiao-xia☆, Studying for doctorate, Department of Histoembryology, Capital Medical University, Beijing 100069, China gxxj@sohu.com
  • Supported by:

    the Scientific Research Development Plan of Beijing Municipal Education Bureau, No. KM2003100250898*; Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality, No. PHR201007113*

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Abstract:

BACKGROUND: Gonad hormones are essential for the maintenance of skeletal integrity. The in vitro cultured ovarian cells can secrete estradiol and progesterone. Alginic acid-polylysine-alginic acid microcapsule provides a barrier between the graft and the recipient, thus promoting the survival of heterotransplants.
OBJECTIVE: To explore the survival and secretion functions of allografted microencapsulated ovarian cells in ovariectomized mice and their effect on bone collagen metabolism after ovariectomy (OVX).
METHODS: Ovarian cells separated from female Kunming mice (6 weeks old) were cultured and microencapsulated with alginic acid-polylysine-alginic acid. A total of 24 female Kunming mice (8 weeks old) were randomly divided into three groups (n=8): normal group: OVX was not performed; OVX group: OVX was performed; transplantation group: microencapsulated ovarian cells were transplanted into abdominal cavity after OVX. Estradiol and/or progesterone levels of the medium of microencapsulated ovarian cells and mice serum were determined by radioimmunoassay. Ⅰ type collagen fibers in the bone matrix were showed by Van Gieson staining. The concentrations of hydroxyproline, Ca, and P were measured in the left femurs of mice.
RESULTS AND CONCLUSION: The concentrations of estradiol and progesterone in the culture medium were not significantly different between the cultured ovarian cells and microencapsulated ovarian cells. The serum estradiol concentration at 90 days after transplantation had no significant difference compared with that of normal group, whereas the serum estradiol concentration of the OVX group was significantly lower than that of the normal group. In the transplantation group, the distribution of collagen fibers was similar to that of the normal group determined by Van Gieson staining. In comparison to the normal group, the OVX group had less, thinner trabecular matrix, and fewer collagen fibers, more free trabecular terminals, and a thinner uncalcified osteoid matrix. The concentrations of Hyp, Ca, and P in the left femurs of the OVX group were lower than those of the normal group. Microencapsulated ovarian cells survive well after transplantation and secrete estrogen, and they can prohibit OVX-caused bone collagen fibers catabolism to some degree.

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