Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (3): 404-408.doi: 10.3969/j.issn.1673-8225.2011.03.006

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Blood compatibility evaluation of the spherical porous chitosan microcarrier

Zhang Rui1, Han Bao-san2, Peng Cheng-hong2   

  1. 1Department of Tumor Surgery, First Affiliated Hospital, Inner Mongolia Medical College, Hohhot   010059, Inner Mongolia Autonomous Region, China
    2Organ Transplantation Center of Ruijin Hospital Affiliated to Medical College of Shanghai Jiao Tong University, Shanghai   200025,China
  • Received:2010-11-08 Revised:2010-12-06 Online:2011-01-15 Published:2011-01-15
  • About author:Zhang Rui☆, Doctor, Associate chief physician, Department of Tumor Surgery, First Affiliated Hospital, Inner Mongolia Medical College, Hohhot 010059, Inner Mongolia Autonomous Region, China ruizhang999@sohu.com
  • Supported by:

    the National High-tech Research and Development Plan of China - the "863" Research Project, No. 2007AA02Z487*; the National Natural Science Foundation of China, No. 20074031*, 30672043*, 30772105*

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Abstract:

BACKGROUND: Literature reported that most of microcarrier was solid and macroporous. Although surface area was significantly increased compared with two-dimensional microcarrier, it was far away from ideal three-dimensional environment.
OBJECTIVE: To construct spherical porous chitosan microcarrier, and to evaluate the blood compatibility of the spherical porous chitosan microcarrier by hemolysis, coagulation, platelet count, platelet aggregation tests.
METHODES: The concentration of 1%, 2%, and 3% spherical porous chitosan microcarrier were successfully constructed using liquid nitrogen freeze-drying technology. Healthy, adult, New Zealand Rabbits were selected, the blood compatibility of spherical porous chitosan microcarrier was evaluated by hemolysis, coagulation, platelet count, platelet aggregation tests.
RESULTS AND CONCLUSION: The concentration of 1%, 2%, and 3% spherical porous chitosan microcarrier hemolysis rate was1.56%, 2.07%, 2.31%, less than 5%, and without hemolyticus. 3 spherical porous chitosan microcarrier concentrations of sample materials had no significant effect on rabbit blood time. There was no significant difference between 3 concentration of spherical porous chitosan microcarrier and sodium chloride negative control group (P > 0.05). 3 spherical porous chitosan microcarrier concentrations of sample materials had no significant effect on platelet count. There was no significant difference between the comparison of leaching liquor injection before and after and intragroup comparison (P > 0.05). The results have confirmed that the spherical porous chitosan microcarrier has no hemolyticus, coagulation, and platelet aggregation. It shows that the spherical porous chitosan microcarrier has good blood compatibility.

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