Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (45): 8382-8385.doi: 10.3969/j.issn.1673-8225.2010.45.006

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In vitro cultivation and multi-inducing differentiation of rabbit bone marrow mesenchymal stem cells

Zhang Rong 1,2, Shen Kai-jin3, Liu Yan-pu2   

  1. 1 Department of Maxillofacial Surgery, 3Emergency Department, Urumchi General Hospital of Lanzhou Military Area Command of Chinese PLA, Urumchi   830000, Xinjiang Uygur Autonomous Region, China;2 Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Fourth Military Medical University of Chinese PLA, Xi’an   710032, Shaanxi Province, China
  • Online:2010-11-05 Published:2010-11-05
  • Contact: Liu Yan-pu, Doctor, Chief physician, Doctoral supervisor, Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shaanxi Province, China
  • About author:Zhang Rong★, Master, Attending physician, Department of Maxillofacial Surgery, Urumchi General Hospital of Lanzhou Military Area Command of Chinese PLA, Urumchi 830000, Xinjiang Uygur Autonomous Region, China;Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shaanxi Province, China zlaq2000@yahoo.com.cn

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Abstract:

BACKGROUND: There is still no unified standard and final conclusion of isolation, culture and evaluation of bone marrow mesenchymal stem cells (BMSCs).
OBJECTIVE: To observe the biological characteristics and multi-differentiation potential of BMSCs through in vitro cultivation, purification, and amplification.
METHODS: BMSCs were isolated and cultured by adherence screening method, followed by observation of proliferation and growth characteristics, for drawing growth curves; inverted phase contrast microscope, hemotoxylun and eosin staining, and alkaline phosphatase staining were used to observe the cell morphology; BMSCs were induced to differentiate into osteoblasts, chondrocytes and vascular endothelial cells, which were identified by calcified nodules-Alizarin red, toluidine blue and Ules europaeus agglutinin (UEA) staining respectively.
RESULTS AND CONCLUSION: By the method of adherence screening, BMSCs were found in stable characteristic, appeared morphologically in spindle and colony-shape, expressed weakly positive of Alkaline phosphatase staining. BMSCs presented with the corresponding cytomorphology and cytobiology characteristics after inducing cultivation of osteoblasts, chondrocytes and vascular endothelial cells. It revealed that adherence screening method is relatively simple and feasible. Furthermore, BMSCs have a good ability of proliferation and could multi-differenrate under suitable condition.

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