Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (44): 8237-8240.doi: 10.3969/j.issn.1673-8225.2010.44.017

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Investigation of normal values of T-lymphocyte subsets in healthy Dai adolescents and adults

Su Ying-zhen 1,2 , Yu Shou-yi2, Jia Man-hong1, Yang Li1, Shi Yu-hua1, Chen Ling1, Yang Chao-jun1, Ma Yan-ling1　   

  1. 1 Center for AIDS/ATDs Prevention and Control, Yunnan Center for Disease Control and Prevention, Kunming   650022, Yunnan Province, China; 2 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou    510515, Guangdong Province, China　
  • Online:2010-10-29 Published:2010-10-29
  • Contact: Ma Yan-ling, Master, Associate chief technician, Center for AIDS/ATDs Prevention and Control, Yunnan Center for Disease Control and Prevention, Kunming 650022, Yunnan Province, China mayanling68@yahoo.com.cn
  • About author:Su Ying-zhen☆, Studying for doctorate, Technician, Center for AIDS/ATDs Prevention and Control, Yunnan Center for Disease Control and Prevention, Kunming 650022, Yunnan Province, China; Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, Guangdong Province, China suyingzhen@163.com
  • Supported by:

     the Branch Subject of Science and Technology Development Program of Guangzhou, No. 2006Z1-E0091*

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Abstract:

BACKGROUND: Under healthy status, T-lymphocyte subsets and other immunocyte maintain stable condition in quantity. As the normal value of T-lymphocyte subsets is influenced by age, gender and race, it may induce infectious disease or autoimmune disease. Therefore, establishment of normal value is important for identifying immune function, disease development and recovery of various diseases.
OBJECTIVE: To investigate the normal value of peripheral blood T-lymphocyte subsets in healthy adolescents and adults.
METHODS: Peripheral blood was collected from 392 Dai healthy adults and adolescents and stained with monoclonal antibodies supplied by Multi TEST four colors T lymphocyte kit. The samples were tested and analyzed by FACS Calibur.
RESULTS AND CONCLUSION: The absolute counting mean of the T-lymphocyte subsets and reference ranges of 82 healthy Dai adolescent subjects were as follows: Lymphocytes 3 054×106 (1 691×106-4 845×106)/L; CD3+ cells 2 055×106 (1 183×106-  3 280×106)/L; CD4+cells 1 072×106 (583×106-1 666×106)/L; CD8+ cells 825×106 (381×106-1 665×106)/L, and the ratio of CD4+ /CD8+ was 1.38 (0.44-2.36). The absolute mean of the T-lymphocyte subsets and reference ranges of 310 healthy adults subjects were as follows: Lymphocytes 2 649×106 (1 243×106-4 754×106)/L; CD3+ 1 763×106 (828×106-3 115×106)/L; CD4+985×106 (450×106-1 708×106)/L; CD8+ 663×106 (275×106-1 335×106)/L; the ratio of CD4+ /CD8+ was 1.64 (0.74-2.00). The absolute numbers of lymphocyte subsets and CD8+ of male were significantly more than the female, while the percentage of CD3+ and CD4+of male was remarkably less than the female (P < 0.05). except of CD4+T-lymphocyte, lymphocyte number, T-lymphocyte absolute number, CD8+T cells absolute number and percentage in healthy people of each age stage were different, in particular, CD8+T cells absolute number and percentage in adolescents were greater than other age groups, and decreased with aging. Compared with healthy populations in other regions, the absolute numbers of CD4+T cells in healthy Dai minority were greater. This study established immunophenotyping of lymphocyte and T cell subsets reference ranges for healthy Dai populations.

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