Abstract:

BACKGROUND: The existing experimental results showed that cholera toxin can induce dendritic cell maturation. Cholera toxin B is non-toxic subunit of cholera toxin, which is a good adjuvant and antigen delivery vector, whether it can promote dendritic cell maturation has not yet been confirmed.
OBJECTIVE: To observe the dendritic cell phenotype and function of 2.4 changes in the initial study of cholera toxin B on dendritic cell maturation. 
METHODS: Dendritic cell 2.4 was proliferated by cell culture. Dendritic cells were divided into five groups: control group: there was no treatment; cholera toxin B 0.1, 1.0, 10 mg/L groups: cholera toxin B with the respective final concentrations were added in the culture medium; positive control group: lipopolysaccharide, with final concentration of 1 mg/L, was added into the culture medium. There were 6 holes in each group. Expression of 2.4 cell surface CD80, CD86, MHC-Ⅱ molecule in dendritic cells cultured at different times were detected by flow cytometry, followed by parallel in vitro mixture with lymphocyte reaction. Types of dendritic cells on T cells with stimulation capacity were observed by MTT. 
RESULTS AND CONCLUSION: 10 mg/L cholera toxin B significantly enhanced dendritic cell surface molecules (CD80, CD86 and MHC-Ⅱ) expression (P < 0.01), and significantly improved the dendritic cells to T cell activation capacity (P < 0.01). There were significant differences between positive control group and blank control group (P < 0.01). Cholera toxin B can enhance the activation of dendritic cells mature, its immune function from the positive regulatory role.