Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (42): 7873-7877.doi: 10.3969/j.issn.1673-8225.2010.42.020

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Periodontal tissue engineered scaffold materials fabricated with zein

Liu Jian, Wu Jing-jing, Li Na, Yang Feng-ying, Xu Yan-zhi   

  1. Department of Stomatology, the Fourth Hospital of Hebei Medical University, Shijiazhuang   050011, Hebei Province, China
  • Online:2010-10-15 Published:2010-10-15
  • Contact: Xu Yan-zhi, Doctor, Professor, Department of Stomatology, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China xu_yanzhi@163.com
  • About author:Liu Jian★, Master, Attending physician, Department of Stomatology, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China Zjh888fff@yahoo.cn
  • Supported by:

    General Project of the National Natural Science Foundation of China, No.30873289*

Abstract:

BACKGROUND: Zein with a unique solubility, heat resistance, film-forming, antimicrobial and antioxidant activities, can be used for moisture proofing, oxygen barrier, anti-UV, anti-static electricity. It has become a source of medical and biotechnology raw materials.
OBJECTIVE: To fabricate zein scaffolds and to explore the suitability of zein as scaffolds for periodontal tissue engineering.
METHODS: Zein porous scaffold was made using the solvent casting/particulate leaching method. Scanning electron microscopy was used to observe the fracture surface and the pore size of zein scaffold. The porosity of zein scaffold was measured by liquid displacement method. Human periodontal ligament cells were cultured in vitro using method of tissue piece, then cultured with different concentrations of extracted liquid of zein. MTT assay was used to calculated the relative growth rate of periodontal cells at 24, 48, 72 hours and to evaluate the cytotoxicity. At the third passage, the cell suspension was incubated on zein scaffold at a density of 3.5×107/L.
RESULTS AND CONCLUSIONS: Zein scaffold displayed a sponge-like structure with a high porosity and sufficient thickness. The porosity and pore size of zein scaffold can be controlled by changing the porogen particles dosage and size. Zein porosity was up to 64.1%-78.0% and zein pores were well-distributed, interconnected, and opened. Human periodontal ligament cells cultured with extracted liquid of zein have good activities. The toxicity of zein scaffold was graded as 0-1. Periodontal ligament cells displayed the full stretching and vigorous growth under scanning electronic microscope. All these prompted that zein scaffold fabricated using the solvent casting/particulate leaching method has high porosity, opened pore wall structure, and good cell compatibility.

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