Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (42): 7819-7823.doi: 10.3969/j.issn.1673-8225.2010.42.008

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Laryngeal defects repair using acellular dermal matrix and Ni-Ti alloy stent in a canine model

Zeng Bin1, Chen Liang-si2, Li Xiang1, Chen Guo-ping1, Peng Jie-ren1   

  1. 1 Department of Otolaryngology-Head and Neck Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou   510120, Guangdong Province, China; 2 Department of Otolaryngology-Head and Neck Surgery, Guangdong People’s Hospital, Guangzhou   510080, Guangdong Province, China　
  • Online:2010-10-15 Published:2010-10-15
  • Contact: Peng Jie-ren, Chief physician, Professor, Department of Otolaryngology-Head and Neck Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China pjrwcx@163.com
  • About author:Zeng Bin★, Studying for master’s degree, Department of Otolaryngology-Head and Neck Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China zb2000190@163.com
  • Supported by:

    a grant by Guangdong Provincial Science and Technology Bureau, No. 2005B10401048*; the Natural Science Foundation of Guangdong Province, No. A2007009*

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Abstract:

BACKGROUND: Ni-Ti alloy stent can be prepared into thyroid cartilage V-shape, and effectively repair the spatial three-dimensional structure, ensure the maximum ventilation throat and vocal function recovery.
OBJECTIVE: To investigate the feasibility of applying acellular dermal matrix (ADM) and Ni-Ti alloy stent to repair laryngeal impairment in canine model. 
METHODS: Subtotal laryngectomy was performed in six healthy adult hybrid canines, which were divided into two groups with 3 dogs in each group. The ADM and Ni-Ti alloy stent were used to repair laryngeal skeleton of the canine in experiment group. In the control group, only Ni-Ti alloy stent was used to repair the laryngeal impairment. Animals were performed X-ray and endoscopic examination in 1 and 6 months after surgery, and were sacrificed at 6 months. The specimens were taken out for gross observation and histological examination.
RESULTS AND CONCLUSION: The result of X-ray showed the Ni-Ti alloy stent was firmly attached to the reduced thyroid cartilage, without migration or extrusion. Gross anatomy examination revealed good repair effect of laryngeal mucosa in experimental group, except a small amount of scar formed in one case, the remaining 2 cases had no scar formation, with smooth throat and without laryngostenosis, postoperative breathing and swallowing function recovered well. In the control group, stent surface granulation and scar tissue proliferated in three cases, 1 patient of which died of dyspnea at 63 days after operation. Histological examination showed most of the epithelium in the experimental group was covered with stratified squamous epithelium, vascular tissue and some lymphocytes were seen in stroma. In the control group, granulation tissue and fibroplasia were evident, without the stratified squamous epithelium covered, there were massive lymphocytes and plasma cells in stroma. Ni-Ti alloy stent combined with ADM could effectively repair the laryngeal impairment. The method provides adequate support and reduces formation of granulation and scars.

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