Abstract:

BACKGROUND: Polylactic acid is a commonly used material for vascular stent coating at present, with advantages of biodegradation, plasticity, low antigenicity and good compatibility. Magnetized metal bare stent may significantly reduce the thrombus and restenosis.
OBJECTIVE: To analyze the cell biocompatibility of a new stent coating composite material, rapamycin-polylactide nano-magnetic microspheres, and investigate the influence on vascular smooth muscle cells proliferation.
METHODS: Small patches of composite material were made of rapamycin-polylactide nano-magnetic microspheres by dispersion polymerization under standard conditions, then were co-cultured with 3-5 passages of rat aortic smooth muscle cells for 7 days, MTT assay was applied to determine the growth and proliferation of smooth muscle cells.
RESULTS AND CONCLUSION: Poly-lactic acid composite membrane of different magnetic microspheres concentrations have different degrees of inhibition on the smooth muscle cell proliferation (P < 0.05). There was no significant difference in the inhibition effect between rapamycin-polylactide nano-magnetic composite microspheres and rapamycin alone (P > 0.05). But the inhibition on the smooth muscle cell proliferation was stronger than single magnetic microspheres of polylactic acid composite membrane (P < 0.05). Composite materials of rapamycin-polylactide nano-magnetic microspheres cam significantly inhibit proliferation of vascular smooth muscle cells, also have good cell biocompatibility.